Acetyl-CoA Carboxylase 2 Antibody [E20M20]

製品コード：F3039

印刷

生物学的記述

Specificity Acetyl-CoA Carboxylase 2 Antibody [E20M20] detects endogenous levels of total Acetyl-CoA Carboxylase 2 protein.
Background Acetyl-CoA Carboxylase 2 (ACC2) is a biotin-dependent carboxylase enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, primarily regulating fatty acid oxidation in oxidative tissues like heart and skeletal muscle. ACC2 features a multi-domain architecture including a biotin carboxylase (BC) domain that carboxylates biotin using ATP and bicarbonate, a biotin carboxyl carrier protein (BCCP) domain that shuttles the carboxyl group, and a carboxyltransferase (CT) domain that transfers it to acetyl-CoA. A unique N-terminal hydrophobic sequence targets and anchors ACC2 to the outer mitochondrial membrane, positioning it near carnitine palmitoyltransferase 1 (CPT1). ACC2 operates as a dimer or filament in its active form, with activity modulated by phosphorylation. Mitochondrially localized malonyl-CoA produced by ACC2 allosterically inhibits CPT1, preventing long-chain fatty acid entry into mitochondria for beta-oxidation during energy-replete states. This mechanism maintains metabolic balance by suppressing fat breakdown when synthesis predominates, with ACC2 knockout enhancing oxidation and insulin sensitivity. ACC2 dysregulation contributes to metabolic disorders, including obesity, type 2 diabetes, and cardiovascular disease.

使用情報

Application WB, IP Dilution
WB IP
1:1000 1:50
Reactivity Human
Source Rabbit Monoclonal Antibody MW 280 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

References

  • https://pubmed.ncbi.nlm.nih.gov/10677481/
  • https://pubmed.ncbi.nlm.nih.gov/35359351/

Application Data