Actinomycin D (Dactinomycin)

製品コードS8964 バッチS896401

印刷

化学情報

Chemical Structure Synonyms Act D, RASP-101 Storage
(From the date of receipt)		 3 years -20°C(in the dark) powder
化学式

C62H86N12O16
分子量 1255.42 CAS No. 50-76-0
Solubility (25°C)* 体外 DMSO 100 mg/mL (79.65 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Actinomycin D (Dactinomycin, Act D, RASP-101) is a significant polypeptide antibiotic isolated from soil bacteria of the genus Streptomyces. Actinomycin D (Dactinomycin) inhibits DNA repair and rests the cell cycle at G1 phase with IC50 of 0.42 μM and 0.4 nM, respectively. Actinomycin D is an autophagy activator, induces p53-independent cell death and prolongs survival in high-risk chronic lymphocytic leukemia.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.
in vitro

Actinomycin D (Dactinomycin) markedly reduces the SMC proliferation via the inhibition of BrdU incorporation at 80 nM. This is further supported by the G1-phase arrest using a flowcytometric analysis. The protein expression levels of proliferating cell nuclear antigen (PCNA), focal adhesion kinase (FAK), and Raf are all suppressed by this compound. Extracellular signalregulated kinases (Erk) involved in cell-cycle arrest are found to be increased by it.[1]
in vivo

Active in two different mouse models that are characterized by either unmutated B-cell receptor or inactive p53 function—both known negative prognostic factors in CLL—Actinomycin D (Dactinomycin) targets survival proteins TOSO, BCL2 and MCL1.[3]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 A10 cells (Vascular SMC)
濃度 80 nM, 800 nM, 8 μM
反応時間 18-24 h
実験の流れ

Actinomycin D (Dactinomycin) is dissolved in 0.1% DMSO, and a vehicle control containing DMSO is also included. Cultured SMC are starved for 24 h followed by incubation with various doses of this compound at 37℃. Drug treatment is carried out for 18-24 h.
動物実験 動物モデル C57BL/6 wild-type mice engrafted with tumor cells from Em-TCL-1 transgenic mice
投薬量 0.06 mg/kg
投与方法 IP

参考

  • https://pubmed.ncbi.nlm.nih.gov/15959627/
  • https://pubmed.ncbi.nlm.nih.gov/9450493/
  • https://pubmed.ncbi.nlm.nih.gov/22743622/

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人間や獣医の診断であるか治療的な使用のためにでない。

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