Alda 1

製品コードS5800 バッチS580001

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₁₅H₁₁Cl₂NO₃
	分子量	324.16	CAS No.	349438-38-6
Solubility (25°C)*	体外	DMSO	65 mg/mL (200.51 mM)
		Ethanol	12 mg/mL (37.01 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Alda 1 is an ALDH2 agonist that activates catalysis for both wild-type ALDH2 (ALDH21) and ALDH22.
in vitro	Alda-1 alleviated liver injury after hepatic I/R. Pretreatment with Alda-1 significantly alleviated I/R-induced elevations of alanine aminotransferase and aspartate aminotransferase, and significantly blunted the pathological injury of the liver. Moreover, Alda-1 significantly inhibited ROS and proinflammatory cytokines production, 4-HNE and MDA accumulation, and apoptosis. Increased ALDH2 activity was found after Alda-1 administration. No significant changes in ALDH2 expression were observed after I/R^[2].

製品説明

Alda 1 is an ALDH2 agonist that activates catalysis for both wild-type ALDH2 (ALDH2*1) and ALDH2*2.

in vitro

Alda-1 alleviated liver injury after hepatic I/R. Pretreatment with Alda-1 significantly alleviated I/R-induced elevations of alanine aminotransferase and aspartate aminotransferase, and significantly blunted the pathological injury of the liver. Moreover, Alda-1 significantly inhibited ROS and proinflammatory cytokines production, 4-HNE and MDA accumulation, and apoptosis. Increased ALDH2 activity was found after Alda-1 administration. No significant changes in ALDH2 expression were observed after I/R^[2].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	normal rat hepatocyte cell (BRL-3A)
	濃度	20 μM
	反応時間	1 hour
	実験の流れ	The H/R (Hypoxia/reoxygenation) group cells are incubated under a hypoxic atmosphere (95% N2+5% CO2) for 6 h followed by 120 min reoxygenation (95% air + 5% CO2). The control incubation is normoxic conditions (95% air + 5% CO2) for the entire experiment. In the H/R+Alda-1 group, cells are incubated with Alda-1 (20 μM) for 60 min at 37℃ (95% air/5% CO2) before exposure to hypoxia. Furthermore, BRL-3A cells are treated with 5 μM 4-HNE for 60min before exposure to hypoxia to estimate whether 4-HNE could affect H/R.
動物実験	動物モデル	Male Sprague-Dawley rats (IR model)
	投薬量	10 mg/kg
	投与方法	i.p.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

The Effects of Alda-1 Treatment on Renal and Intestinal Injuries After Cardiopulmonary Resuscitation in Pigs [ Front Med (Lausanne), 2022, 9:892472]	PubMed: 35646953

The Effects of Alda-1 Treatment on Renal and Intestinal Injuries After Cardiopulmonary Resuscitation in Pigs [ Front Med (Lausanne), 2022, 9:892472]

PubMed: 35646953

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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