Datasheet

生物学的記述

Specificity	α-adaptin A Antibody [D21J17] detects endogenous levels of total α-adaptin A protein.
Background	α-Adaptin is a crucial subunit of the adaptor protein complex 2 (AP-2), which belongs to the family of adaptor protein complexes involved in intracellular vesicle transport. α-adaptin forms part of the heterotetrameric AP-2 complex, which consists of two large subunits (α and β2), a medium subunit (μ2), and a small subunit (σ2). The α-adaptin subunit contains a C-terminal appendage domain that functions as a scaffolding platform for endocytic accessory proteins. It also possesses regions that bind polyphosphoinositide-containing lipids, positioning AP-2 on the plasma membrane. AP-2 operates primarily in clathrin-dependent endocytosis by linking clathrin lattices to cargo proteins and membrane lipids and facilitating the formation of clathrin-coated vesicles (CCVs). The α and σ subunits contribute to recognizing specific endocytosis signal motifs, such as [ED]-X-X-X-L-[LI], which enables selective cargo capture. AP-2 also plays roles in recycling synaptic vesicle membranes, post-endocytic trafficking, and possibly ARF6-regulated non-clathrin pathways. Dysfunction or mutation in AP-2 components, such as α-adaptin, disrupts vesicle formation and endocytosis.

Specificity

α-adaptin A Antibody [D21J17] detects endogenous levels of total α-adaptin A protein.

Background

α-Adaptin is a crucial subunit of the adaptor protein complex 2 (AP-2), which belongs to the family of adaptor protein complexes involved in intracellular vesicle transport. α-adaptin forms part of the heterotetrameric AP-2 complex, which consists of two large subunits (α and β2), a medium subunit (μ2), and a small subunit (σ2). The α-adaptin subunit contains a C-terminal appendage domain that functions as a scaffolding platform for endocytic accessory proteins. It also possesses regions that bind polyphosphoinositide-containing lipids, positioning AP-2 on the plasma membrane. AP-2 operates primarily in clathrin-dependent endocytosis by linking clathrin lattices to cargo proteins and membrane lipids and facilitating the formation of clathrin-coated vesicles (CCVs). The α and σ subunits contribute to recognizing specific endocytosis signal motifs, such as [ED]-X-X-X-L-[LI], which enables selective cargo capture. AP-2 also plays roles in recycling synaptic vesicle membranes, post-endocytic trafficking, and possibly ARF6-regulated non-clathrin pathways. Dysfunction or mutation in AP-2 components, such as α-adaptin, disrupts vesicle formation and endocytosis.

使用情報

Application

WB, IP, IHC, IF, FCM

Dilution

WB	IF	FCM
1:200	1:1000-1:2000	1:2000

Reactivity

Bovine, Hamster, Human, Mouse, Non-human primate, Rat

Source

Mouse Monoclonal Antibody

MW

~100 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/31353312/
https://pubmed.ncbi.nlm.nih.gov/8491205/

α-adaptin A Antibody [D21J17]

生物学的記述

使用情報

References

Application Data