alpha-Asarone

製品コードS4772 バッチS477201

印刷

化学情報

Chemical Structure Synonyms trans-Asarone Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C12H16O3
分子量 208.25 CAS No. 2883-98-9
Solubility (25°C)* 体外 DMSO 41 mg/mL (196.87 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Alpha (α)-asarone (trans-Asarone) is one of the main pharmacologically active compounds present in Acorus calamus Linn (Acoraceae), Acorus tatarinowii Schott (Acoraceae), and Acorus gramineus Solander. It has anticonvulsant, neuroprotective, anxiolytic and nootropic effects.
in vitro α-asarone possesses a endothelium-dependent vasodilation property, and in normal HUVEC-derived EA.hy926 cells, α-asarone has been shown to upregulate the expression of phosphorylated eNOS (Ser1177), causing an increase in the production of NO. It protects against injury to endothelial cells by attenuating Ang II-mediated changes in intracellular ROS/NO[1].
in vivo Alpha-Asarone treatment alleviates locomotor hyperactivity in Fmr1 KO mice. It improves m1 mAChR expression and up-regulated the ACh levels in the striatum. Its treatment also attenuates the increased striatal AChE activity in Fmr1 KO mice. Alpha-asarone has been reported to have a beneficial effectin cognitive impairment induced by dysfunction of the cholinergicsystem in the brain through inhibition of AChE activity[2]. Of all the doses tested (2, 10, 40, 80 and 120 mg/kg) in rats, 10 mg/kg α-Asarone improves the quality of sleep, as indicated by an increased NREM bout duration, reduced arousal index, and decreased bout frequencies of NREM sleep and wakefulness. A marginal decrease in the hypothalamic and body temperatures is also observed. Higher doses, on the other hand, not only reduce the quantity and quality of sleep, but also produce hypothermia[3].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 EA.hy926 cell line
濃度 10, 50, 100 µmol/L
反応時間 1 h
実験の流れ EA.hy926 cells are subcultured at 50,000/well in 96-well plates as in the experiment above and pretreated with α-asarone at different concentrations (10, 50, and 100 μmol/L) for 1 h. Subsequently, Ang II (0.1 μmol/L) is added to the cultures and incubated for 24 h at 37°C. Thereafter, the cells are loaded with the fluorescent probes for 30 min, and then the extracellular residual dyes are washed away before detection.
動物実験 動物モデル Fmr1 knockout (KO) mice (male, 6–8 weeks of age)
投薬量 10 mg/kg
投与方法 i.p.

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。