α-Hederin

製品コードS3914 バッチS391401

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₄₁H₆₆O₁₂

分子量

750.96

CAS No.

27013-91-8

Solubility (25°C)*

体外

DMSO

100 mg/mL (133.16 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	16.000mg/ml (21.31mM)	Taking the 1 mL working solution as an example, add 50 μL of 320 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	4.000mg/ml (5.33mM)	Taking the 1 mL working solution as an example, add 50 μL of 80 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	α-hederinは、溶血作用およびアポトーシス作用を示す水溶性の五環性トリテルペノイドサポニンです。
in vitro	α-hederin shows strong inhibitory activity on the growth of breast cancer cells and induces apoptosis in these cells. α-hederin induces depolarization of mitochondrial membrane potential which releases Apaf-1 and cytochrome c from the intermembrane space into the cytosol, where they promote caspase-3 and caspase-9 activation.
in vivo	After a single i.v. (2 mg/kg) administration, the mean plasma clearance (CL), volume of distribution (V_SS) and elimination half-life (t_1/2) of α-hederin in rtas are 0.24 L/h/kg, 0.25 L/kg and 2.67 h, respectively. The oral bioavailability (F) of α-hederin in rats is about 0.14%, which might result from the poor intestinal absorption and/or extensive biliary excretion. α-Hederin decreases the hepatotoxicity of cadmium in mice by inducing hepatic metallothionein I/II, and the mechanism partly involved the upregulation of metal-lothionein expression mediated by TNF-α and IL-6.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	The human breast cancer cell lines MCF-7 and MDA-MB-231
	濃度	0.08, 0.4, 2 and 10 μg/ml
	反応時間	12, 24 and 48 h
	実験の流れ	The MTT assay is used to measure the inhibition of growth by α-hederin in breast cancer cell lines. Briefly, 5×10³ cells are seeded into a 96-well plate in triplicate and 8 h later α-hederin is added into the wells at the indicated final concentrations (0.08, 0.4, 2 and 10 μg/ml), while cells cultured in medium with 0.05% DMSO as a negative control. After incubation with α-hederin for 12, 24 and 48 h, the medium in each well is replaced with 20 μl of MTT at 5 mg/ml final concentration, and 4 h later 150 μl DMSO/well is added to dissolve the formed violet formazan crystals within metabolically viable cells. The plates are incubated at room temperature for 15 min and then read at 490 nm with a microplate reader. The percentage of growth inhibition is calculated as (OD of the control-OD of the experiment samples)/OD of the control × 100.
動物実験	動物モデル	Sprague-Dawley (SD) rats
	投薬量	10 mg/kg (p.o.) or 2 mg/kg (i.v.)
	投与方法	p.o. or i.v.

参考

https://pubmed.ncbi.nlm.nih.gov/24842044/
http://pubs.rsc.org/en/content/articlelanding/2015/ay/c4ay02837f#!divAbstract

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。