(+)-α-Lipoic acid

製品コードS3998 バッチS399801

化学情報

	Synonyms	(R)-(+)-α-Lipoic acid, α-Lipoic acid, Alpha-Lipoic acid	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₈H₁₄O₂S₂
	分子量	206.33	CAS No.	1200-22-2
Solubility (25°C)*	体外	DMSO	41 mg/mL (198.71 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	(+)-α-Lipoic acid ((R)-(+)-α-Lipoic acid, α-Lipoic acid, Alpha-Lipoic acid), a physiological form of thioctic acid, is a strong antioxidant that relieves diabetic neuropathic symptoms. It shows superior antioxidative effects to its racemate. R(+)-α-lipoic acid is an essential cofactor of mitochondrial enzyme complexes. R(+)-α-lipoic acid inhibits NF-κB-dependent HIV-1 LTR activation.
in vitro	Using L6 myotubes and 3T3-L1 adipocytes as a model of muscle and fat cells in culture, R(+)-α-lipoic acid (R-LA) is shown to increase tyrosine phosphorylation of insulin receptor and IRS-1, to enhance PI 3-kinase and Akt1 activities, to elevate GLUT4 content in the plasma membranes, and to increase glucose uptake into the cells. 3T3-L1 adipocytes possess a low capacity to reduce R-α-lipoic acid and the oxidized isoforms are effective in stimulating glucose transport. R-α-lipoic acid modulates glucose transport by changing intracellular redox status. Insulin receptor is a target of R-LA action^[1].

製品説明

(+)-α-Lipoic acid ((R)-(+)-α-Lipoic acid, α-Lipoic acid, Alpha-Lipoic acid), a physiological form of thioctic acid, is a strong antioxidant that relieves diabetic neuropathic symptoms. It shows superior antioxidative effects to its racemate. R(+)-α-lipoic acid is an essential cofactor of mitochondrial enzyme complexes. R(+)-α-lipoic acid inhibits NF-κB-dependent HIV-1 LTR activation.

in vitro

Using L6 myotubes and 3T3-L1 adipocytes as a model of muscle and fat cells in culture, R(+)-α-lipoic acid (R-LA) is shown to increase tyrosine phosphorylation of insulin receptor and IRS-1, to enhance PI 3-kinase and Akt1 activities, to elevate GLUT4 content in the plasma membranes, and to increase glucose uptake into the cells. 3T3-L1 adipocytes possess a low capacity to reduce R-α-lipoic acid and the oxidized isoforms are effective in stimulating glucose transport. R-α-lipoic acid modulates glucose transport by changing intracellular redox status. Insulin receptor is a target of R-LA action^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	3T3-L1 preadipocytes
	濃度	250 μM
	反応時間	2-48 h
	実験の流れ	Cells seeded in 12-well plates were incubated for 2–48 h with R-LA (250 μM) and loaded with H2DCF-DA (20 μM) for the last 30 min in PBS containing R-LA. For some experiments cells were washed and incubated with R-LA (0.25-1 μM) and H2DCF-DA (20 μM) for 30 min. Following the treatments, cells were gently scraped by a lifter and suspended in the same media. For detection of intracellular fluorescence, cells were excited using a 488-nm argon-ion laser in a flow cytometer. The dichlorofluorescein (DCF) emission was recorded at 530 nm. Data were collected from at least 20,000 cells.

参考

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。