Alpinetin

製品コードS3875 バッチS387501

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₁₆H₁₄O₄
	分子量	270.28	CAS No.	36052-37-6
Solubility (25°C)*	体外	DMSO	54 mg/mL (199.79 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Alpinetin, a composition of Alpinia katsumadai Hayata, has been reported to have a number of biological properties, such as antibacterial, antitumor and other important therapeutic activities.
in vitro	Alpinetin markedly inhibits the LPS- induced TNF-α, IL-6 and IL-1β production both in vitro and vivo. Furthermore, alpinetin blocks the phosphorylation of IκBα protein, p65, p38 and extracellular signal-regulated kinase (ERK) in LPS stimulated RAW 264.7 cells^[1]. Alpinetin can suppress the proliferation and invasiveness of GSCs and induce apoptosis in GSCs^[2].
in vivo	Alpinetin attenuates lung histopathologic changes in mouse models^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Raw 264.7 cells
	濃度	0–300 μg/ml
	反応時間	1 h
	実験の流れ	Cell viability is evaluated by MTT assay. Briefly, 100 μl cells are plated at a density of 4×10⁵ cells/ml in 96-well plates in a 37 ℃, 5% CO2 incubator for 1 h. Then the cells are treated with 50 μl of alpinetin of different concentrations (0-300 μg/ml) for 1 h, followed by stimulation with 50 μl LPS (4 μg/ml). The total volume of each well is 200 μl, and the concentrations of Alpinetin and LPS are four times diluted finally. After 18 h of LPS stimulation, 20 μl MTT (5 mg/ml) is added to each well, and the cells are further incubated for an additional 4 h. The supernatant is removed and the formation of formazan is resolved with 150 μl/well of DMSO. The optical density is measured at 570 nm on a microplate reader.
動物実験	動物モデル	Male BALB/c mice
	投薬量	50 mg/kg
	投与方法	i.p.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Inhibitory Effect of Tangeretin and Cardamonin on Human Intestinal SGLT1 Activity In Vitro and Blood Glucose Levels in Mice In Vivo [ Nutrients, 2021, 13(10)3382]	PubMed: 34684383

Inhibitory Effect of Tangeretin and Cardamonin on Human Intestinal SGLT1 Activity In Vitro and Blood Glucose Levels in Mice In Vivo [ Nutrients, 2021, 13(10)3382]

PubMed: 34684383

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。