Ambroxol HCl

製品コードS3064 バッチS306402

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C13H18Br2N2O.HCl
分子量 414.56 CAS No. 23828-92-4
Solubility (25°C)* 体外 DMSO 3 mg/mL warmed with 50ºC water bath (7.23 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 AmbroxolHCl is a potent inhibitor of the neuronal Na+ channels, inhibits TTX-resistant Na+ currents with IC50 of 35.2 μM and 22.5 μM for tonic and phasic block, inhibits TTX-sensitive Na+ currents with IC50 of 100 μM. Phase 3.
in vitro Ambroxol inhibits Na+ channels in sensory neurons. The potency for tonic block of TTX-r channels is relatively high. Ambroxol affects the Na+ current kinetics of TTX-r and TTX-s channels differently. In CNaIIA cells, the compound behaves like a charged local anesthetic: the block is dependent on stimulus number and increases with higher frequencies in a train of depolarizing stimuli. In CNaIIA cells, ambroxol inhibits inactivated channels 5.5-fold more potently than resting channels. The corresponding factor for TTX-r channels is only 3.3.[1] Ambroxol inhibits the release of histamine, leukotrienes and cytokines from human leukocytes and mast cells. [2]
in vivo Ambroxol inhibited histamine release by more than 50% from human adenoidal mast cells (1000 microM ambroxol) and skin mast cells (100 microM ambroxol) stimulated by Con A and compound 48/80, respectively. Ambroxol (100 microM) strikingly inhibited anti-IgE induced release of both histamine, LTC4, IL-4 and IL-13 from basophils and reduced both histamine and LTB4 release induced by C5a or Zymosan in monocytes. The drug also reduced LTB4 and superoxide anion production in granulocytes stimulated by zymosan or fMLP.

プロトコル(参考用のみ)

キナーゼアッセイ Electrophysiology.
Na+ currents are recorded in the whole-cell, voltage-clamp configuration at room temperature. The extracellular solution contains 140.0 mM NaCl, 5.3 mM KCl, 27.0 mM glucose, 10.0 mM HEPES, 0.8 mM MgCl2, and 1.8 mM CaCl2, pH 7.4. In most of the experiments on DRG neurons, 300 nM tetrodotoxin (TTX) is added. The intracellular medium consists of 50.0 mM CsCl, 90.0 mM CsF, 10.0 mM NaF, 10.0 mM HEPES, 10.0 mM EGTA, and 2.0 mM MgCl2, pH 7.4. Recording pipettes have resistances of 0.8 to 1.8 MΩ when filled with intracellular solution. Currents are recorded using an EPC-9 amplifier.

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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