受注:045-509-1970 |
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Synonyms | Carboxymethoxylamine hemihydrochloride, AOAA hemihydrochloride, Aminooxyacetate hemihydrochloride | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C2H5NO3.1/2HCl |
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分子量 | 109.3 | CAS No. | 2921-14-4 | ||||||||
Solubility (25°C)* | 体外 | DMSO | 22 mg/mL (201.28 mM) | ||||||||
Water | 22 mg/mL (201.28 mM) | ||||||||||
Ethanol | 6 mg/mL (54.89 mM) | ||||||||||
体内 (毎回新しく調製した物を用意してください) |
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | AOA (Aminooxyacetic acid) hemihydrochloride is an inhibitor of aminobutyrate aminotransferase involved in amino acid and polyamine metabolism. AOA hemihydrochloride is also a CBS (cystathionine β-synthase) inhibitor. |
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in vitro | Aminooxyacetic acid (AOAA) is a malate-aspartate shuttle inhibitor, leading to decreased intracellular ATP levels and altering cell cycle and increasing apoptosis and necrosis of C6 glioma cells, without affecting the survival of primary astrocyte cultures. AOAA also decreases the glycolytic rate and the levels of extracellular lactate and pyruvate, without affecting the mitochondrial membrane potential of C6 cells. Aminooxyacetic acid can selectively inhibit the proliferation of breast cancer cells[1]. |
in vivo | Aminooxyacetic acid suppresses the growth of breast adenocarcinoma in athymic mice[1]. AOAA is much less toxic in rats than in mice[2]. |
細胞アッセイ | 細胞株 | C6 glioma cells |
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濃度 | 0.1, 0.25, 0.5, 1, 5 mM | |
反応時間 | 24 h | |
実験の流れ | C6 glioma cells are plated into 24-well or 12-well cell culture plates at the initial density of 1 × 106 cells/mL in Dulbecco's Modified Eagle's Medium containing 4500 mg/L D-glucose, 584 mg/L L-glutamine, and 1% penicillin and streptomycin, supplemented with 10% fetal bovine serum. The cells are maintained in a 5% CO2 incubator at 37℃. Cell survival is assessed 24 h after AOAA treatment by intracellular LDH assay. |
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動物実験 | 動物モデル | female Wistar rats |
投薬量 | 15 to 100 mg/kg | |
投与方法 | i.p. or s.c. |
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NRF2 activation by cysteine as a survival mechanism for triple-negative breast cancer cells [ Research Square, 2024, 10.21203/rs.3.rs-3849836/v1] | PubMed: none |
Dysregulated Gln-Glu-α-ketoglutarate axis impairs maternal decidualization and increases the risk of recurrent spontaneous miscarriage [ Cell Rep Med, 2023, 4(5):101026] | PubMed: 37137303 |
Metabolic rewiring in keratinocytes by miR-31-5p identifies therapeutic intervention for psoriasis [ EMBO Mol Med, 2023, e15674.] | PubMed: 36855912 |
The mitochondrial NADH shuttle system is a targetable vulnerability for Group 3 medulloblastoma in a hypoxic microenvironment [ Cell Death Dis, 2023, 14(11):784] | PubMed: 38036520 |
L-cystathionine protects against oxidative stress and DNA damage induced by oxidized low-density lipoprotein in THP-1-derived macrophages [ Front Pharmacol, 2023, 14:1161542] | PubMed: 37560474 |
Glutamine Metabolism Is Required for Alveolar Regeneration during Lung Injury [ Biomolecules, 2022, 12(5)728] | PubMed: 35625656 |
Glutamate dehydrogenase 1 mediated glutaminolysis sustains HCC cells survival under glucose deprivation [ J Cancer, 2022, 13(3):1061-1072] | PubMed: 35154470 |
Yap-Myc signaling induces pancreatic stellate cell activation through regulating glutaminolysis [ Exp Cell Res, 2022, 411(1):113000] | PubMed: 34958764 |
Mistimed H 2 S Upregulation, Nrf2 Activation and Antioxidant Proteins Levels in Renal Tubular Epithelial Cells Subjected to Anoxia and Reoxygenation [ Biomed Rep, 2020, 13(2):3] | PubMed: 32509306 |
Glutamate dehydrogenase 1 mediated glutaminolysis sustains HCC cells proliferation and survival under glucose deprivation [ Research Square, 2020, 10.21203/rs.3.rs-105979/v1] | PubMed: None |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。