Anacardic Acid

製品コードS7582 バッチS758203

印刷

化学情報

Synonyms

6-pentadecylsalicylic Acid

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₂H₃₆O₃

分子量

348.52

CAS No.

16611-84-0

Solubility (25°C)*

体外

DMSO

69 mg/mL (197.98 mM)

Ethanol (warmed with 50ºC water bath)

69 mg/mL (197.98 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	4.250mg/ml (12.19mM)	Taking the 1 mL working solution as an example, add 50 μL of 85 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Anacardic Acid（6-ペンタデシルサリチル酸）は、p300およびp300/CBP結合因子ヒストンアセチルトランスフェラーゼの強力な阻害剤であり、抗菌活性、抗微生物活性、プロスタグランジン合成酵素阻害、チロシナーゼおよびリポキシゲナーゼ阻害作用も有しています。
in vitro	Anacardic acid is found to be a common inhibitor of several clinically targeted enzymes such as NFkB kinase, histone acetyltransferase (HATs), lipoxygenase (LOX-1), xanthine oxidase, tyrosinase and ureases. Anacardic acid is also an efficient inhibitor of Tip60 in vitro.It inhibits the Tip60-dependent acetylation and activation of the ATM protein kinase in HeLa cells, and sensitizes the cells to the cytotoxic effects of radiation. Anacardic acid can effectively prevent cell damage induced by H2O2 because this can be converted to ROS, hydroxyl radicals, in the presence of metal ions.
in vivo	Anacardic acid attenuates over-expression of Gata4, α-MHC and cTnT in fetal mouse hearts exposed to ethanol. Anacardic acid inhibits binding of P300, PCAF to the promoter of Gata4.

プロトコル(参考用のみ)

キナーゼアッセイ	HAT and HDAC Activity Assays
キナーゼアッセイ	After homogenization of cardiac tissues, nucleoproteins are extracted using a Nuclear Extract Kit according to the manufacturer's instructions. HAT and HDAC activities of the nuclear protein extracts are determined using a colorimetric assay included in the HAT and HDAC assay kits.
細胞アッセイ	細胞株	HeLa and 293T cells, SQ20B and SCC35 squamous cell carcinoma cell lines
	濃度	0-100 μM
	反応時間	40 min
	実験の流れ	HeLa and 293T cells are grown in Dulbecco’s modified Eagle’s medium/10% fetal calf serum. SQ20B and SCC35 squamous cell carcinoma cell lines are cultured in modified eagles medium/10% fetal calf serum.For anacardic acid (EMD Biosciences, CA) exposure, cells are preincubated with anacardic acid for 40 min, irradiated, then allowed to recover for 4 h. Cells are then switched to fresh media and allowed to grow for 10 days.
動物実験	動物モデル	9 to 11 week old Kunming mice
	投薬量	0, 1.25, 2.5, 5, 10 mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/12624111/
https://pubmed.ncbi.nlm.nih.gov/16844118/
https://pubmed.ncbi.nlm.nih.gov/25101666/

https://pubmed.ncbi.nlm.nih.gov/22103711/

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カスタマーフィードバック

: Data from [Data independently produced by , , Front Immunol, 2017, 7:696]

: Data from [Data independently produced by , , Oncol Rep, 2017, 38(1):229-236]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Local soft niches in mechanically heterogeneous primary tumors promote brain metastasis via mechanotransduction-mediated HDAC3 activity [ Sci Adv, 2025, 11(9):eadq2881]	PubMed: 40009679
PTBP1 crotonylation promotes colorectal cancer progression through alternative splicing-mediated upregulation of the PKM2 gene [ J Transl Med, 2024, 22(1):995]	PubMed: 39497094
Fluoride Alters Gene Expression via Histone H3K27 Acetylation in Ameloblast-like LS8 Cells [ Int J Mol Sci, 2024, 25(17)9600]	PubMed: 39273544
DNAJC12 downregulation induces neuroblastoma progression via increased histone H4K5 lactylation [ J Mol Cell Biol, 2024, mjae056]	PubMed: 39716470
The mechanics of local niches in the primary tumor regulate breast cancer brain metastasis [ POLYU ELECTRONIC THESES, 2023, viii, 164]	PubMed: None
HBV covalently closed circular DNA minichromosomes in distinct epigenetic transcriptional states differ in their vulnerability to damage [ Hepatology, 2021, 10.1002/hep.32245]	PubMed: 34779008
Caspase-8 Regulates the Antimyeloma Activity of Bortezomib and Lenalidomide [ J Pharmacol Exp Ther, 2021, 379(3):303-309]	PubMed: 34588172
Anacardic acid induces IL-33 and promotes remyelination in CNS [ Proc Natl Acad Sci U S A, 2020, 202006566]	PubMed: 32817520
Histone Acetyltransferase (HAT) P300/CBP Inhibitors Induce Synthetic Lethality in PTEN-Deficient Colorectal Cancer Cells Through Destabilizing AKT [ Int J Biol Sci, 2020, 25;16(11):1774-1784]	PubMed: 32398948
Histone acetyltransferase promotes fluoride toxicity in LS8 cells. [ Chemosphere, 2020, 247:125825]	PubMed: 31927229

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。