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Synonyms | Ang-(1-7), Angiotensin fragment 1-7 | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C41H62N12O11 |
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分子量 | 899.00 | CAS No. | 51833-78-4 | |
Solubility (25°C)* | 体外 | Water | 50 mg/mL (55.61 mM) | |
DMSO | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Angiotensin (1-7) (Ang-(1-7), Angiotensin fragment 1-7) is a bioactive component of the renin-angiotensin system that is formed endogenously from either Ang I or Ang II. Angiotensin (1-7) is a canine ACE inhibitor with an IC50 of 0.65 μM and inhibits the activity mediated by myostatin through Mas receptor. |
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in vitro | Angiotensin (1-7) prevents the decrease of the myotube diameter and MHC Levels Induced by myostatin in C2C12 cells. Angiotensin (1-7) decreases the myostatin-dependent increment of E3 ubiquitin ligases and ROS, the NF-κB signaling induced by myostatin, the myostatin-dependent activity through the mas receptor and Akt/PKB activity in C2C12 myotubes.[1] |
in vivo | Daily Angiotensin (1-7) administration significantly reduces colitis severity observable at both gross and histological levels in DSS treated mice. The anti-inflammatory effects of Angiotensin (1-7) treatment are associated with a reduction in the phosphorylated forms of p38 MAPK, ERK1/2 and Akt post DSS induction, the anti-inflammatory properties of Angiotensin (1-7) are in part mediated through reduction of Ang II levels.[3] |
細胞アッセイ | 細胞株 | C2C12 cells |
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濃度 | 10 nM | |
反応時間 | 30 min, 60 min | |
実験の流れ | The C2C12 cells are differentiated until day 5 by changing DMEM–FSB to DMEM supplemented with horse serum. The myotubes are incubated with 1 µg/ml of human recombinant myostatin and/or 10 nM of Angiotensin (1-7). The myotubes are treated with 10 µM of MK2206 for Akt/PKB inhibition, or 10 µM of A779 antagonist for Mas receptor inhibition. In brief, the procedure is as follows: differentiated cells are pre-treated with Angiotensin (1-7) for 30 or 60 min, depending on the experiments, and then treated with myostatin. |
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動物実験 | 動物モデル | DSS treated mice |
投薬量 | 0.01 mg/kg, 0.06 mg/kg, 0.1 mg/kg, 0.3 mg/kg, 1 mg/kg | |
投与方法 | i.p. |
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ACE2 negatively regulates the Warburg effect and suppresses hepatocellular carcinoma progression via reducing ROS-HIF1α activity [ Int J Biol Sci, 2023, 19(8):2613-2629] | PubMed: 37215979 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
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