Anisomycin

製品コードS7409 バッチS740906

印刷

化学情報

Chemical Structure Synonyms Flagecidin, Wuningmeisu C Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C14H19NO4
分子量 265.3 CAS No. 22862-76-6
Solubility (25°C)* 体外 DMSO (warmed with 50ºC water bath) 53 mg/mL (199.77 mM)
Ethanol (warmed with 50ºC water bath) 53 mg/mL (199.77 mM)
Water 2 mg/mL (7.53 mM)
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Anisomycin (Flagecidin, Wuningmeisu C) is a bacterial antibiotic isolated from Streptomyces griseolus, which inhibits protein synthesis, and also act as a JNK activator. Anisomycin upregulates autophagy and increases apoptosis.
in vitro Anisomycin (3 μM) decreases protein synthesis in MDA16 and MDA-MB-468 cells, and reduces colony formation by MDA-MB-468 cells. This compound causes an increase in the number of apoptotic cells in MDA-MB-468 cultures, but not in MDA16 cultures. It actives JNK phosphorylation in MDA-MB-468 cells.[2] In U251 and U87 cells, this chemical (0.01-8 μM) inhibits the cell growth in time- and concentration-dependent manners with the IC50 (48 h) values of 0.233 and 0.192 μmol/L, respectively. It (4 μM) causes 21.5% and 25.3% of apoptosis proportion in U251 and U87 cells, respectively, and activates p38 MAPK and JNK, while inactivated ERK1/2. This compound (4 μM) reduces the level of PP2A/C subunit in a time-dependent manner in U251 and U87 cells.[3] It inhibits EAC cell proliferation in concentration-dependent manner.[4]
in vivo Peritumoral administration of anisomycin (5 mg/kg) significantly suppresses Ehrlich ascites carcinoma (EAC) growth resulting in the survival of approximately 60% of the mice 90 days after EAC inoculation.[4]

プロトコル(参考用のみ)

キナーゼアッセイ JNK phosphorylation
500,000 cells/well are seeded in 6-well plates and incubated overnight. Cells are then incubated for 1 h with test compounds or DMSO as vehicle control (final concentration 1% v/v). Puromycin is added (final concentration of 18 μM) and cells incubated for a further 10 min to label nascent polypeptide chains. Background labelling is determined by incubating cells without puromycin. Cells are then washed in HBSS, harvested by scraping and centrifuged (300 g, 5 min). Cells are resuspended in 0.5 mL 50 mM DTT containing phosphatase inhibitors and incubated at 95℃ for 10 min. Samples are then snap frozen in liquid nitrogen and stored at -20℃ until blotted. Samples (20–30 μg protein/sample) are blotted onto a PVDF membrane. The membrane is blocked and incubated with anti-phospho-Thr183/Tyr185-JNK antibody overnight at 4℃. Secondary antibodies are used to label the primary antibody and detected using an infrared scanner. The intensity of the fluorescence signal for anti-phospho-JNK antibody is background corrected and normalized for loading.
細胞アッセイ 細胞株 Ehrlich ascites carcinoma (EAC) cells
濃度 500 ng/mL
反応時間 48 h
実験の流れ For the assay, EAC cells are plated in 96-well plates at a density of 10,000 cells/well/200 µL of medium. The cells are treated with the different concentrations of this compound for 48 h. Adriamycin (500 ng/mL) is used as a positive control. 0.5 mg/mL of MTT is added to each well. 4 h later, the formazan product of MTT reduction is dissolved in DMSO, and absorbance is measured at 570 nm using a Model 680 microplate reader.
動物実験 動物モデル Male BALB/c mice
投薬量 5 mg/kg
投与方法 peritumorally

参考

  • https://pubmed.ncbi.nlm.nih.gov/9154836/
  • https://pubmed.ncbi.nlm.nih.gov/24333448/
  • https://pubmed.ncbi.nlm.nih.gov/22684030/
  • https://pubmed.ncbi.nlm.nih.gov/23525555/

カスタマーフィードバック

, , J Cell Biochem, 2017, 118(12):4905-4913

Data from [Data independently produced by , , Redox Biol, 2018, 14:59-71]

Data from [Data independently produced by , , Redox Biol, 2018, 14:576-587]

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UFMylation safeguards human hepatocyte differentiation and liver homeostasis by regulating ribosome dissociation [ Cell Rep, 2025, 44(5):115686] PubMed: 40347470
sFRP5 ameliorates atherosclerosis by suppressing the JNK/TLR9 pathway in macrophages [ Transl Res, 2025, 281:1-13] PubMed: 40409587
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Promoter Methylation of WIF1 is Involved in IL-17-Induced Chondrocyte Inflammatory Injury and Matrix Degradation via Promoting Wnt5a/MAPK-JNK Signaling [ Mol Biotechnol, 2025, none] PubMed: 40072748
Transcriptional landscape and predictive potential of long noncoding RNAs in peritoneal recurrence of gastric cancer [ Mol Cancer, 2024, 23(1):284] PubMed: 39736670

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人間や獣医の診断であるか治療的な使用のためにでない。

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