Datasheet

生物学的記述

Specificity	Apolipoprotein E Antibody [B14G10] detects endogenous levels of total Apolipoprotein E protein.
Background	Apolipoprotein E (ApoE) is a glycoprotein produced predominantly by hepatocytes and astrocytes, essential for systemic and central nervous system lipid homeostasis. Circulating as three major isoforms, ApoE2, ApoE3, and ApoE4, which differ at residues Cys112 and Arg158, ApoE mediates receptor-dependent uptake, redistribution, and clearance of cholesterol-rich lipoproteins (HDL, VLDL, chylomicron remnants) throughout the body and brain. Lipid-free ApoE adopts a two-domain organization: an N-terminal receptor-binding domain (residues 1–191, forming a four-α-helix bundle with key motifs for LDL receptor family binding) and a C-terminal domain (residues 210–299) comprising amphipathic helices that mediate lipid association, joined by a flexible hinge. Upon lipidation, ApoE undergoes a conformational “domain unlocking,” exposing binding sites and stabilizing discoidal HDL-like particles. Lipidated ApoE directs lipoproteins to receptors such as LDLR, LRP1, CD36, and HSPGs, facilitating endocytosis, lysosomal lipolysis, cholesterol efflux, and esterification pathways critical for membrane repair, steroidogenesis, and neuronal health. In the brain, astrocyte-derived ApoE3 supports amyloid-β (Aβ) oligomerization and clearance via LRP1, and promotes synaptic plasticity through PI3K/Akt signaling. In contrast, the ApoE4 isoform’s structural instability impairs lipidation, enhances Aβ42 aggregation and seeding, increases neuroinflammation through TLR4/NF-κB activation, and disrupts tau phosphorylation and neurotrophic signaling, conferring a dramatically higher risk for Alzheimer’s disease, atherosclerosis, and neurodegeneration. ApoE2, conversely, enhances receptor binding and clearance, offering cardiovascular protection.

Specificity

Apolipoprotein E Antibody [B14G10] detects endogenous levels of total Apolipoprotein E protein.

Background

Apolipoprotein E (ApoE) is a glycoprotein produced predominantly by hepatocytes and astrocytes, essential for systemic and central nervous system lipid homeostasis. Circulating as three major isoforms, ApoE2, ApoE3, and ApoE4, which differ at residues Cys112 and Arg158, ApoE mediates receptor-dependent uptake, redistribution, and clearance of cholesterol-rich lipoproteins (HDL, VLDL, chylomicron remnants) throughout the body and brain. Lipid-free ApoE adopts a two-domain organization: an N-terminal receptor-binding domain (residues 1–191, forming a four-α-helix bundle with key motifs for LDL receptor family binding) and a C-terminal domain (residues 210–299) comprising amphipathic helices that mediate lipid association, joined by a flexible hinge. Upon lipidation, ApoE undergoes a conformational “domain unlocking,” exposing binding sites and stabilizing discoidal HDL-like particles. Lipidated ApoE directs lipoproteins to receptors such as LDLR, LRP1, CD36, and HSPGs, facilitating endocytosis, lysosomal lipolysis, cholesterol efflux, and esterification pathways critical for membrane repair, steroidogenesis, and neuronal health. In the brain, astrocyte-derived ApoE3 supports amyloid-β (Aβ) oligomerization and clearance via LRP1, and promotes synaptic plasticity through PI3K/Akt signaling. In contrast, the ApoE4 isoform’s structural instability impairs lipidation, enhances Aβ42 aggregation and seeding, increases neuroinflammation through TLR4/NF-κB activation, and disrupts tau phosphorylation and neurotrophic signaling, conferring a dramatically higher risk for Alzheimer’s disease, atherosclerosis, and neurodegeneration. ApoE2, conversely, enhances receptor binding and clearance, offering cardiovascular protection.

使用情報

Application

WB, IP, IHC, IF, FCM

Dilution

WB	IP	IHC	IF	FCM
1:1000 - 1:10000	1:20	1:800	1:50	1:20

Reactivity

Human

Source

Rabbit Monoclonal Antibody

MW

36 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/25173806/
https://pubmed.ncbi.nlm.nih.gov/21873229/

Application Data

WB

Validated by Selleck

Lane 1: Human liver, Lane 2: Human cerebellum, Lane 3: HepG2, Lane 4: Human serum