Aprotinin

製品コードS7377 バッチS737708

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C284H432N84O79S7
分子量 6511.51 CAS No. 9087-70-1
Solubility (25°C)* 体外 Water 100 mg/mL (15.35 mM)
DMSO Insoluble
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Aprotinin is a small protein serine protease inhibitor, used to reduce perioperative blood loss and transfusion.
in vitro Aprotinin is an antifibrinolytic molecule that inhibits trypsin and related proteolytic enzymes. In cell biology, aprotinin is used as an enzyme inhibitor to prevent protein degradation during lysis or homogenization of cells and tissues. In the presence of aprotinin, the fibrinolytic activity is inhibited concentration dependently and the coagulation time is prolonged. Aprotinin is an effective inhibitor of the contact (intrinsic) coagulation pathway in vitro[2].
in vivo Aprotinin inhibits clot lysis in vitro as well as rat-tail bleeding time in vivo and prolongs coagulation time in human plasma. In a rat arteriovenous shunt model, aprotinin reduces thrombus weight [2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 mouse G8-1 and C2C12 skeletal muscle myoblasts
濃度 2 TIU/ml
反応時間 12hrs
実験の流れ

Mouse G8-1 myoblasts are plated DMEM + 20% FBS (maintenance medium), in which they remain undifferentiated. When cells reach approximately 40-50% confluence, different protease inhibitors are added to the culture media and cells are incubated overnight. Cells are then switched to differentiation-promoting media (DMEM + 10% horse serum ± protease inhibitor) and incubated for 7 days.
動物実験 動物モデル Male Wistar rats
投薬量 1.5 mg/kg and 3 mg/kg/h, 3 mg/kg and 6 mg/kg/h up to 5 mg/kg and 10 mg/kg/h
投与方法 bolus injection followed by a maintenance infusion(i.p.)

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

SARS-CoV-2 requires cholesterol for viral entry and pathological syncytia formation [ Elife, 2021, 10e65962] PubMed: 33890572
Acetylation targets HSD17B4 for degradation via the CMA pathway in response to estrone. [ Autophagy, 2017, 13(3):538-553] PubMed: 28296597

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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