APS-2-79 HCl

製品コードS8355 バッチS835501

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C23H21N3O3.HCl
分子量 423.89 CAS No. 2002381-31-7
Solubility (25°C)* 体外 DMSO 97 mg/mL (228.83 mM)
Ethanol 32 mg/mL (75.49 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 APS-2-79 is a MAPK antagonist that modulating KSR-dependent MAPK signalling by antagonizing RAF heterodimerization as well as the conformational changes required for phosphorylation and activation of KSR-bound MEK.
in vitro APS-2-79 functions as an antagonist of MEK phosphorylation by RAF through direct binding of the KSR active site. APS-2-79 is inactive when KSR was absent or when the KSR2(A690F) mutant was used for in vitro assays. APS-2-79 increases the potency of several MEK inhibitors specifically within Ras-mutant cell lines by antagonizing release of negative feedback signalling[1].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HCT-116, A549, SK-MEL-239 and A375 cell lines
濃度 1 μM
反応時間 72 h
実験の流れ Cell viability assays are performed in 96 well plates. Optimal cell densities for 96 well plate assays are determined to obtain linear growth over the timecourse of assays. Specifically, A549, HCT-116, A375, SK-MEL-239, COLO-205, LOVO, SK-MEL-2, CALU-6, MEWO, SW620 and SW1417 cells are plated at 500 cells per well and treated with inhibitors for 72hrs before measuring viability. H2087 and HEPG2 cells are plated at 2000 cells per well, and treated with inhibitors for 72hrs. Cell viability is measured using resazurin, and the percent cell viability is determined by normalizing inhibitor-treated samples to DMSO controls

カスタマーフィードバック

Data from [Data independently produced by , , Experimental and therapeutic medicine, 2018, https://doi.org/10.3892/etm.2018.6081]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Evolutionarily conserved regulation of sleep by epidermal growth factor receptor signaling. [ Sci Adv, 2019, 5(11):eaax4249] PubMed: 31763451
Effect of aromatase inhibitor letrozole on the proliferation of spermatogonia by regulating the MAPK pathway [Wang S, et al. Exp Ther Med, 2018, 15(6):5269-5274] PubMed: 29805545

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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