Datasheet

生物学的記述

Specificity	Artemis Antibody [H20J21] detects endogenous levels of total Artemis protein.
Background	Artemis (DCLRE1C, also known as SNM1C) is a ubiquitously expressed nuclear nuclease belonging to the metallo-β-lactamase (MBL) superfamily, essential for non-homologous end-joining (NHEJ) DNA repair and V(D)J recombination in developing lymphocytes. Artemis is a 692-amino-acid protein with an N-terminal catalytic domain (residues 1-360) comprising a core MBL fold (α/β-β/α sandwich) and embedded β-CASP subdomain containing conserved motifs 1-4 (His33, His35, His115, Asp116 coordinating Zn²⁺ ions M1/M2) and motifs A-C for nucleic acid processing, plus a largely unstructured C-terminal regulatory tail (residues 361-692) that autoinhibits activity and mediates DNA-PKcs tethering. DNA-PKcs phosphorylates Artemis (e.g., at Thr127, Ser251) upon DSB recognition, activating its structure-specific endonuclease activity to open hairpin-sealed coding ends in V(D)J recombination, trim 5'/3' overhangs, flaps, and bubbles in NHEJ for XRCC4/LIG4 ligation, and process IR-induced complex ends while antagonizing HR via 53BP1-PTIP interaction. ATM/ATR further phosphorylate Artemis for G2/M and S-phase checkpoint recovery, promoting Cdk1-cyclin B activation and cyclin E degradation via SCF^{Fbw7}. Biallelic mutations cause radiosensitive severe combined immunodeficiency (RS-SCID) with defective V(D)J joining and genome instability predisposing to malignancy.

Specificity

Artemis Antibody [H20J21] detects endogenous levels of total Artemis protein.

Background

Artemis (DCLRE1C, also known as SNM1C) is a ubiquitously expressed nuclear nuclease belonging to the metallo-β-lactamase (MBL) superfamily, essential for non-homologous end-joining (NHEJ) DNA repair and V(D)J recombination in developing lymphocytes. Artemis is a 692-amino-acid protein with an N-terminal catalytic domain (residues 1-360) comprising a core MBL fold (α/β-β/α sandwich) and embedded β-CASP subdomain containing conserved motifs 1-4 (His33, His35, His115, Asp116 coordinating Zn²⁺ ions M1/M2) and motifs A-C for nucleic acid processing, plus a largely unstructured C-terminal regulatory tail (residues 361-692) that autoinhibits activity and mediates DNA-PKcs tethering. DNA-PKcs phosphorylates Artemis (e.g., at Thr127, Ser251) upon DSB recognition, activating its structure-specific endonuclease activity to open hairpin-sealed coding ends in V(D)J recombination, trim 5'/3' overhangs, flaps, and bubbles in NHEJ for XRCC4/LIG4 ligation, and process IR-induced complex ends while antagonizing HR via 53BP1-PTIP interaction. ATM/ATR further phosphorylate Artemis for G2/M and S-phase checkpoint recovery, promoting Cdk1-cyclin B activation and cyclin E degradation via SCF^{Fbw7}. Biallelic mutations cause radiosensitive severe combined immunodeficiency (RS-SCID) with defective V(D)J joining and genome instability predisposing to malignancy.

使用情報

Application

WB, IP

Dilution

WB	IP
1:1000	1:200

Reactivity

Human, Monkey

Source

Rabbit Monoclonal Antibody

MW

90 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/14744996/
https://pubmed.ncbi.nlm.nih.gov/34387696/

Artemis Antibody [H20J21]

生物学的記述

使用情報

References

Application Data