AS1949490

製品コードE0400 バッチE040001

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₂₀H₁₈ClNO₂S

分子量

371.88

CAS No.

1203680-76-5

Solubility (25°C)*

体外

DMSO

74 mg/mL (198.98 mM)

Ethanol

37 mg/mL (99.49 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	3.7mg/ml (9.95mM)	Taking the 1 mL working solution as an example, add 50 μL of 74 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.53mg/ml (1.43mM)	Taking the 1 mL working solution as an example, add 50 μL of 10.6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	AS1949490, a competitive inhibitor of 5'-lipid phosphatase SHIP2 with IC50 values of 0.62 µM and 0.34 µM for human (Ki value is 0.44 µM) and mouse respectively, promotes protein kinase C-dependent stabilization of brain-derived neurotrophic factor mRNA in cultured cortical neurons.
in vitro	AS1949490 increases the phosphorylation of Akt, glucose consumption and glucose uptake in L6 myotubes. In FAO hepatocytes, this compound suppresses gluconeogenesis.^[1]
in vivo	Acute administration of AS1949490 inhibites the expression of gluconeogenic genes in the livers of normal mice. Chronic treatment of diabetic db/db mice with this compound significantly loared the plasma glucose level and improved glucose intolerance.^[1]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	L6 myotubes, FAO hepatocytes
	濃度	1-16 µM
	反応時間	15 min, 24 h, 48 h
	実験の流れ	L6 myotubes are incubated for 15 min both with and without AS1949490 treatment before being stimulated with insulin at indicated concentration and 1 nM or fetal bovine serum at indicated concentrations for 10 min. Protein lysates from L6 myotubes are immunoblotted with anti-phosphorylated Akt antibody or anti-Akt antibody. FAO cells are treated with this compound and insulin for 24 h. The glucose concentration in the medium is measured after incubation for 6 h in gluconeogenesis buffer.
動物実験	動物モデル	Six week old male ICR mice, C57BL/KsJ Jcl-dbm +db/+db mice
	投薬量	300 mg/kg
	投与方法	o.g.

参考

https://pubmed.ncbi.nlm.nih.gov/19694723/

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。