ATF-6 Antibody [G8H4]

製品コード：F4203

印刷

生物学的記述

Specificity ATF-6 (D4Z8V) Rabbit mAb detects endogenous levels of total ATF-6 protein.
Background ATF-6 (Activating Transcription Factor 6) is a type II transmembrane glycoprotein belonging to the bZIP transcription factor family, residing in the endoplasmic reticulum (ER) membrane as a key sensor of the unfolded protein response (UPR). ATF6 features an N-terminal cytoplasmic domain with a basic leucine zipper (bZIP) DNA-binding motif and transcriptional activation regions facing the cytosol, a single transmembrane helix, and a C-terminal ER luminal domain that associates with BiP/GRP78 under homeostatic conditions. Upon ER stress from unfolded protein accumulation, BiP dissociates from ATF6, enabling its trafficking to the Golgi apparatus for sequential cleavage by site-1 protease (S1P) and site-2 protease (S2P), which liberates the active N-terminal cytoplasmic fragment. This cleaved fragment translocates to the nucleus where it binds ER stress response elements (ERSE) to directly activate transcription of UPR target genes, including GRP78/BiP, GRP94, protein disulfide isomerase (PDI), XBP1, and CHOP, enhancing ER chaperone capacity, protein folding, ER-associated degradation (ERAD), and ER biogenesis to restore proteostasis and promote cell survival. Dysregulation of ATF6 contributes to diabetes through impaired insulin signaling, neurodegeneration via protein aggregation, and cancer progression.

使用情報

Application WB, IP Dilution
WB IP
1:1000 1:50
Reactivity Human, Mouse
Source Rabbit Monoclonal Antibody MW 90-100 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

References

  • https://pubmed.ncbi.nlm.nih.gov/10564271/
  • https://pubmed.ncbi.nlm.nih.gov/39570676/

Application Data

WB

Validated by Selleck

  • F4203-wb.gif
    Lane 1: 293T, Lane 2: Hela, Lane 3: Jurkat, Lane 4: K562