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Synonyms | Adenosine-Triphosphate, Adenosine 5'-triphosphate | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C10H16N5O13P3 |
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分子量 | 507.18 | CAS No. | 56-65-5 | |
Solubility (25°C)* | 体外 | Water | 100 mg/mL (197.16 mM) | |
DMSO | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | ATP (Adenosine-Triphosphate, Adenosine 5'-triphosphate) is a multifunctional nucleoside triphosphate and an important endogenous signaling molecule in immunity and inflammation. |
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in vitro | ATP, a multifunctional nucleoside triphosphate and an important endogenous signaling molecule, restores signaling responses to anti-TCR treatment in Jurkat cells.[2] |
in vivo | ATP can activate the NLRP3 inflammasome and enhance the production of pro-inflammatory cytokines, thus protecting mice against bacterial infection.[3] |
細胞アッセイ | 細胞株 | Jurkat cells |
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濃度 | 50 mM | |
反応時間 | -- | |
実験の流れ | Cells are washed with calcium-free and magnesium-free 1×DPBS to remove calcium and magnesium. Then cells are suspended with 450 µL 1×DPBS into a 1.5 mL centrifugal tube, treated with 50 mL Streptolysin O (0.5 µg/ml), and incubated 60 mins at 37℃. Next, cells are centrifuged at 400 g for 4 mins to discard the supernatant. Cells are re-suspended with 100 mL 1×DPBS, treated with 11 µL 50 mM ATP, and incubated in the 37℃ for indicated time. |
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動物実験 | 動物モデル | Kunming mice |
投薬量 | 50 mg/kg | |
投与方法 | i.p. |
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The ARID1A-METTL3-m6A axis ensures effective RNase H1-mediated resolution of R-loops and genome stability [ Cell Rep, 2024, 43(2):113779] | PubMed: 38358891 |
Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing [ Cell Rep Med, 2023, 4(8):101129] | PubMed: 37480849 |
CD39 pathway inhibits Th1 cell function in tuberculosis [ Immunology, 2022, 10.1111/imm.13493] | PubMed: 35574713 |
ATRA-mediated-crosstalk between stellate cells and Kupffer cells inhibits autophagy and promotes NLRP3 activation in acute liver injury [ Cell Signal, 2022, 93:110304] | PubMed: 35278669 |
NAD+ supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells [ Cell Rep, 2021, 36(6):109516] | PubMed: 34380043 |
Necroptosis Underlies Neutrophilic Inflammation Associated with the Chronic Rhinosinusitis with Nasal Polyps (CRSwNP) [ J Inflamm Res, 2021, 14:3969-3983] | PubMed: 34429629 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。