Datasheet

生物学的記述

Specificity	ATP1B1 Antibody [A23C23] detects endogenous levels of total ATP1B1 protein.
Background	ATP1B1, the β1 regulatory subunit of the Na+/K+-ATPase within the P-type ATPase β-subunit family, forms obligate heterotetramers with α1-4 catalytic subunits to establish transmembrane electrochemical gradients essential for excitable tissues and epithelial vectorial transport. Its single-pass transmembrane helix and large extracellular domain stabilize α-subunit maturation, plasma membrane trafficking, and voltage-sensitive conformational cycling between E1P (Na+-occluded) and E2P (K+-occluded) states, while allosterically modulating Na+ affinity and ouabain sensitivity through direct interactions with the α-subunit actuator domain. Circadian regulation via CLOCK:BMAL1 binding to promoter E-boxes drives rhythmic Atp1b1 expression antiphasic to blood pressure, where DEC1 repression lowers β1 levels to elevate vascular tone; conversely, DEC1 deficiency enhances pump density and reduces hypertension through sustained membrane hyperpolarization. In renal distal tubules, ATP1B1 coordinates paracellular Cl- permeability via modulation of claudin-2/4/7 expression, while cardiac β1 sustains diastolic relaxation through optimized sarcolemmal K+ influx during repolarization. Rare mutations disrupt α-β assembly, causing familial hemiplegic migraine, while heterozygous variants associate with PCOS through insulin resistance and granulosa cell dysfunction.

Specificity

ATP1B1 Antibody [A23C23] detects endogenous levels of total ATP1B1 protein.

Background

ATP1B1, the β1 regulatory subunit of the Na+/K+-ATPase within the P-type ATPase β-subunit family, forms obligate heterotetramers with α1-4 catalytic subunits to establish transmembrane electrochemical gradients essential for excitable tissues and epithelial vectorial transport. Its single-pass transmembrane helix and large extracellular domain stabilize α-subunit maturation, plasma membrane trafficking, and voltage-sensitive conformational cycling between E1P (Na+-occluded) and E2P (K+-occluded) states, while allosterically modulating Na+ affinity and ouabain sensitivity through direct interactions with the α-subunit actuator domain. Circadian regulation via CLOCK:BMAL1 binding to promoter E-boxes drives rhythmic Atp1b1 expression antiphasic to blood pressure, where DEC1 repression lowers β1 levels to elevate vascular tone; conversely, DEC1 deficiency enhances pump density and reduces hypertension through sustained membrane hyperpolarization. In renal distal tubules, ATP1B1 coordinates paracellular Cl- permeability via modulation of claudin-2/4/7 expression, while cardiac β1 sustains diastolic relaxation through optimized sarcolemmal K+ influx during repolarization. Rare mutations disrupt α-β assembly, causing familial hemiplegic migraine, while heterozygous variants associate with PCOS through insulin resistance and granulosa cell dysfunction.

使用情報

Application

WB, IP, IHC, IF

Dilution

WB	IHC
1:1000-1:10000	1:200

Reactivity

Human, Mouse, Sheep, Pig, Dog

Source

Mouse Monoclonal Antibody

MW

35 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/30012868/
https://pubmed.ncbi.nlm.nih.gov/10411917/

Application Data

WB

Validated by Selleck

Lane 1: Hela, Lane 2: MCF7, Lane 3: U251