Datasheet

生物学的記述

Specificity	ATP6V1B2 Antibody [K16G16] detects endogenous levels of total ATP6V1B2 protein.
Background	ATP6V1B2 serves as the B2 isoform of the V1 sector in vacuolar H+-ATPase (V-ATPase) complexes, which function as ATP-driven proton pumps to acidify intracellular compartments like lysosomes and endosomes, as well as certain plasma membranes. It forms part of the peripheral V1 domain's catalytic hexamer alongside A subunits, featuring nucleotide-binding sites that drive rotational ATP hydrolysis to power proton translocation through the membrane-embedded V0 sector. ATP6V1B2-containing V-ATPases interact directly with the Ragulator-Rag GTPase complex on lysosomal surfaces, sensing amino acid availability to trigger mTORC1 recruitment and activation; upon leucine or arginine stimulation, V-ATPase proton pumping generates a localized acidic microenvironment that stabilizes RagA/B in GTP-bound states, facilitating mTORC1 docking via Raptor and subsequent phosphorylation of S6K1/4EBP1 to promote anabolic translation while inhibiting catabolism. This proton gradient also couples lysosomal biogenesis with nutrient signaling, where ATP6V1B2 mutations impair acidification, disrupt autophagic flux, and paradoxically sustain mTORC1 activity by altering v-ATPase disassembly-reassembly dynamics. ATP6V1B2 maintains cellular homeostasis in nutrient-fluctuating environments by coordinating lysosomal degradation with growth decisions, making it ideal for researchers studying amino acid sensing or macropinocytosis, where v-ATPase inhibition reveals mTORC1 dependency. Dysregulation through somatic mutations activates autophagy while preserving mTORC1, contributing to B-cell lymphoma survival under leucine scarcity, whereas variants causing distal renal tubular acidosis demonstrate impaired acid-base homeostasis.

Specificity

ATP6V1B2 Antibody [K16G16] detects endogenous levels of total ATP6V1B2 protein.

Background

ATP6V1B2 serves as the B2 isoform of the V1 sector in vacuolar H+-ATPase (V-ATPase) complexes, which function as ATP-driven proton pumps to acidify intracellular compartments like lysosomes and endosomes, as well as certain plasma membranes. It forms part of the peripheral V1 domain's catalytic hexamer alongside A subunits, featuring nucleotide-binding sites that drive rotational ATP hydrolysis to power proton translocation through the membrane-embedded V0 sector. ATP6V1B2-containing V-ATPases interact directly with the Ragulator-Rag GTPase complex on lysosomal surfaces, sensing amino acid availability to trigger mTORC1 recruitment and activation; upon leucine or arginine stimulation, V-ATPase proton pumping generates a localized acidic microenvironment that stabilizes RagA/B in GTP-bound states, facilitating mTORC1 docking via Raptor and subsequent phosphorylation of S6K1/4EBP1 to promote anabolic translation while inhibiting catabolism. This proton gradient also couples lysosomal biogenesis with nutrient signaling, where ATP6V1B2 mutations impair acidification, disrupt autophagic flux, and paradoxically sustain mTORC1 activity by altering v-ATPase disassembly-reassembly dynamics. ATP6V1B2 maintains cellular homeostasis in nutrient-fluctuating environments by coordinating lysosomal degradation with growth decisions, making it ideal for researchers studying amino acid sensing or macropinocytosis, where v-ATPase inhibition reveals mTORC1 dependency. Dysregulation through somatic mutations activates autophagy while preserving mTORC1, contributing to B-cell lymphoma survival under leucine scarcity, whereas variants causing distal renal tubular acidosis demonstrate impaired acid-base homeostasis.

使用情報

Application

WB

Dilution

WB

1:1000

Reactivity

Human, Mouse, Rat, Monkey

Source

Rabbit Monoclonal Antibody

MW

55 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/39757940/
https://pubmed.ncbi.nlm.nih.gov/30720463/

Application Data

WB

Validated by Selleck

Lane 1: 293, Lane 2: ACHN, Lane 3: COS-7, Lane 4: vero