Atractylenolide I

製品コードS8291 バッチS829101

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₁₅H₁₈O₂

分子量

230.30

CAS No.

73069-13-3

Solubility (25°C)*

体外

DMSO (warmed with 50ºC water bath)

46 mg/mL (199.73 mM)

Ethanol (warmed with 50ºC water bath)

46 mg/mL (199.73 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Atractylenolide I is the major sesquiterpenoid of the rhizome of A. macrocephala and shows a wide spectrum of pharmacological activities such as antiinflammatory, digestion promoting, and antioxidant effects.
in vitro	Atractylenolide I decreases the TNF-α level in LPS-stimulated peritoneal macrophages in a dosedependent manner, IC50=23.1 μM. It also inhibits NO production in LPS-activated peritoneal macrophages, IC50=41 μM. The IC50 of this compound for inhibiting the activity of iNOS is 67.3 μM. It shows no marked effects against 5-lipoxygenase (5-LOX) and cyclooxygenase (COX). This chemical is also reported previously to act on white blood cell membranes and TLR4 and its antiinflammatory activity is related to antagonizing TLR4 ^[1].
in vivo	Atractylenolide I has anti-inflammatory effects. The treatment of this compound significantly attenuates LPS-induced lung wet-to-dry weight ratio and MPO activity in LPS-induced acute lung injury mouse. It also significantly inhibits the production of TNF-α, IL-6, IL-1β, IL-13, and MIF production in bronchoalveolar lavage fluid (BALF), as well as neutrophils and macrophages in BALF, upregulates the production of IL-10 in BALF. This chemical protects mice acute lung injury induced by LPS via inhibition of TLR4 expression and NF-κB activation^[2].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	peritoneal macrophages
	濃度	1-1000 μM
	反応時間	24 h
	実験の流れ	2 × 10⁴ cells per well are plated into 96-well plates, cells/well to a final volume of 200 μL, incubated at 37 °C for 24 h, and given a fresh change of medium. The cells are treated with atractylenolide I (1-1000 μM) dissolved in DMSO, and with LPS (final concentration 10 μg/mL) alone or in combination with this compound (1–100 μM) dissolved in DMSO. This DMSO percentage allows the optimal solubilization of this chemical in aqueous solution. The control and LPS wells receive the same amount of DMSO. After 24 h of incubation at 37 °C, 20 μL of MTT (5 mg/mL) is added to each well and 4 h later the cells are lysed with 150 μL DMSO. The plate is shaken for 10 min and 30 min later the optical densities (OD570) for this compound are compared with the OD of the control or LPS stimulated wells to assess the cytotoxicity.
動物実験	動物モデル	LPS-induced acute lung injury mouse
	投薬量	5, 10 and 20 mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/17221938/
https://pubmed.ncbi.nlm.nih.gov/26297303/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Atractylenolide-I Sensitizes Triple-Negative Breast Cancer Cells to Paclitaxel by Blocking CTGF Expression and Fibroblast Activation [ Front Oncol, 2021, 11:738534]	PubMed: 34692516
Atractylenolide‑1 alleviates gastroparesis in diabetic rats by activating the stem cell factor/c‑kit signaling pathway [ Mol Med Rep, 2021, 24(4)691]	PubMed: 34368880
Atractylenolide I Induces Apoptosis and Suppresses Glycolysis by Blocking the JAK2/STAT3 Signaling Pathway in Colorectal Cancer Cells. [ Front Pharmacol, 2020, 26;11:273]	PubMed: 32273843
Lactobacillus Protects Against S. Typhimurium-Induced Intestinal Inflammation by Determining the Fate of Epithelial Proliferation and Differentiation. [ Mol Nutr Food Res, 2020, 64(5):e1900655]	PubMed: 31953989

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。