Azoramide

製品コードS8304 バッチS830401

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₁₅H₁₇ClN₂OS

分子量

308.83

CAS No.

932986-18-0

Solubility (25°C)*

体外

DMSO

61 mg/mL (197.51 mM)

Ethanol

61 mg/mL (197.51 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution

5%DMSO 1 Corn oil

3.05mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 61 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Azoramide is a small-molecule modulator of the unfolded protein response (UPR). It improves ER protein-folding ability and activates ER chaperone capacity to protect cells against ER stress.
in vitro	Azoramide may have the protective effects of enhancing chaperone expression and reducing protein synthesis without inducing cytotoxicity and apoptosis. Azoramide may require the presence of intact IRE1 and PERK branches of the UPR to fully increase chaperone capacity. Azoramide is found to be a kind of compound with the dual property of not only boosting ER folding acutely but also activating ER chaperone capacity chronically to promote ER homeostasis. Its treatment potently protects cells against chemically-induced ER stress conditions. Azoramide preserves beta cell function and survival during metabolic ER stress. Azoramide pretreatment does not impair ER function as part of its initial action. Azoramide treatment leads to increased SERCA expression, resulting in enhanced retention of Ca+2 within the ER. Azoramide interacts with UPR pathways to promote resolution of ER stress and improve ER function^[1].
in vivo	Azoramide improves glucose homeostasis in mice with genetic obesity and diet-induced obesity. Remarkably, azoramide treatment significantly improves insulin sensitivity and glucose tolerance and beta cell function in obese mice in multiple preclinical models^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	INS1 cells
	濃度	20 µM
	反応時間	60 h
	実験の流れ	INS1 cells are treated with 25 mM Glucose and 500 µM Palmitate (G/P) in the absence or presence of 20 µM azoramide for 60 hours. At the end of the incubation, viability is measured using the CellTiter-Glo cell viability assay system.
動物実験	動物モデル	ob/ob mice
	投薬量	150mg/kg
	投与方法	oral

参考

[1] Fu S, et al. Sci Transl Med. 2015, 7(292):292ra98.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Azoramide ameliorates cadmium-induced cytotoxicity by inhibiting endoplasmic reticulum stress and suppressing oxidative stress [ PeerJ, 2024, 12:e16844]	PubMed: 38313032
Exogenous iron impairs the anti-cancer effect of ascorbic acid both in vitro and in vivo [ J Adv Res, 2022, S2090-1232(22)00149-7]	PubMed: 35777727
Azoramide ameliorated tachypacing-induced injury of atrial myocytes differentiated from human induced pluripotent stem cell by regulating endoplasmic reticulum stress [ Stem Cell Res, 2022, 60:102686]	PubMed: 35101669

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。