B02

製品コードS8434 バッチS843401

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₂H₁₇N₃O

分子量

339.39

CAS No.

1290541-46-6

Solubility (25°C)*

体外

DMSO (warmed with 50ºC water bath)

67 mg/mL (197.41 mM)

Ethanol (warmed with 50ºC water bath)

20 mg/mL (58.92 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	3.3mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 66 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	1.6mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 32 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	B02 is a small-molecule inhibitor of human RAD51 with an IC50 of 27.4 μM, but does not inhibit its E. coli homologue RecA (IC50 > 250 μM).
in vitro	B02 is a specific inhibitor of human RAD51 recombinase, blocks HR repair in human embryonic kidney (HEK) and breast cancer cells and increases their sensitivity to a wide range of DNA damaging agents. Also, B02 enhances DNA damage and apoptosis induced by decitabine in MM cells^[1]. B02 shows high specificity for RAD51 and does not significantly inhibit RAD54 in the range of concentrations from 0 to 200 μM^[2]. B02 shows biological effect in human and mouse cells. In human embryonic kidney (HEK) cells, B02 disrupts RAD51 foci formation in response to DNA damage and inhibited DSB repair and DSB-dependent HR. B02 can also increase the sensitivity of cancer cells to chemotherapeutic DNA damaging agents^[3].
in vivo	B02 significantly increases the anti-tumor activity of cisplatin in vivo. B02 is tolerated by mice at doses up to 50 mg/kg without obvious body weight loss. No detectable morphological changes induced by B02 in kidneys and livers, main organs for detoxification are found^[3].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	The human MM cell lines NCI-H929 (H929), RPMI 8226, ARP-1, U266 and MM.1S cells
	濃度	10 μM
	反応時間	1 h
	実験の流れ	Proliferation of MM-cell lines is monitored by the WST-1 colorimetric cell-count assay. MM cell lines are seeded in 96-well plates at ~8000 cells/well. The cells are treated with or without B02 (10 μM) for 1 h, followed by treatment with vehicle (DMSO) or DOX (20-160 nM) for 72 h. WST-1 reagent is added to the culture medium in each well at a 1:10 ratio, and incubation continues at 37°C for 4 h. Relative cell number is estimated from absorbance at 450 nm using a spectrophotometer.
動物実験	動物モデル	NCR nude mice
	投薬量	50 mg/kg
	投与方法	i.p.

参考

カスタマーフィードバック

: Data from [Data independently produced by , , J Virol, 2018, 92(15)]

: Data from [Data independently produced by , , Sci Rep, 2018, 8(1):14421]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

The fragile X locus is prone to spontaneous DNA damage that is preferentially repaired by nonhomologous end-joining to preserve genome integrity [ iScience, 2024, 27(2):108814]	PubMed: 38303711
Selective utilization of non-homologous end-joining and homologous recombination for DNA repair during meiotic maturation in mouse oocytes [ Cell Prolif, 2023, 56(4):e13384]	PubMed: 36564861
Lack of impact of the ALDH2 rs671 variant on breast cancer development in Japanese BRCA1/2-mutation carriers [ Cancer Med, 2023, 12(6):6594-6602]	PubMed: 36345163
RAD52 prevents accumulation of Polα-dependent replication gaps at perturbed replication forks in human cells [ bioRxiv, 2023, 2023.04.12.536536]	PubMed: 37090680
DNA-PKcs promotes fork reversal and chemoresistance [ Mol Cell, 2022, 82-20:3932-3942.e6]	PubMed: 36130596
Targeted Inhibition of DNA-PKcs, ATM, ATR, PARP, and Rad51 Modulate Response to X Rays and Protons [ Radiat Res, 2022, 198(4):336-346]	PubMed: 35939823
cGAS guards against chromosome end-to-end fusions during mitosis and facilitates replicative senescence [ Protein Cell, 2021, 10.1007/s13238-021-00879-y 10.1126/science.abc5386]	PubMed: 34676498
Temporally distinct post-replicative repair mechanisms fill PRIMPOL-dependent ssDNA gaps in human cells [ Mol Cell, 2021, 81(19):4026-4040.e8]	PubMed: 34624216
Control of replication stress and mitosis in colorectal cancer stem cells through the interplay of PARP1, MRE11 and RAD51 [ Cell Death Differ, 2021, 10.1038/s41418-020-00733-4]	PubMed: 33531658
Loss of N-Glycanase 1 Alters Transcriptional and Translational Regulation in K562 Cell Lines [ G3 (Bethesda), 2020, 4;10(5):1585-1597]	PubMed: 32265286

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。