Batimastat (BB-94)

製品コードS7155 バッチS715502

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₃H₃₁N₃O₄S₂

分子量

477.64

CAS No.

130370-60-4

Solubility (25°C)*

体外

DMSO

96 mg/mL (200.98 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	6.000mg/ml (12.56mM)	Taking the 1 mL working solution as an example, add 50 μL of 120 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. Also inhibits the activitity of other metalloproteases, such as ADAM17.
in vitro	Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. ^[1] This compound exhibits an unexpected binding geometry, with the thiophene ring deeply inserted into the primary specificity site. ^[2]
in vivo	Batimastat (BB-94) can inhibit metastatic spread and growth of B16-BL6 murine melanoma. ^[1] In an orthotopic colon tumor model in mice, this compound treatment results in inhibition of primary tumor growth (by 50%), local/regional spread(from 67% to 35%), and distant metastasis(from 30% to 10%).^[3] It reduces in vivo growth of experimental hemangiomas, most probably by blocking endothelial cell recruitment by the transformed cells or by interfering with cell organization in vascular structures. ^[4]

製品説明

Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. Also inhibits the activitity of other metalloproteases, such as ADAM17.

in vitro

Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. ^[1]

This compound exhibits an unexpected binding geometry, with the thiophene ring deeply inserted into the primary specificity site. ^[2]

in vivo

Batimastat (BB-94) can inhibit metastatic spread and growth of B16-BL6 murine melanoma. ^[1]

In an orthotopic colon tumor model in mice, this compound treatment results in inhibition of primary tumor growth (by 50%), local/regional spread(from 67% to 35%), and distant metastasis(from 30% to 10%).^[3]

It reduces in vivo growth of experimental hemangiomas, most probably by blocking endothelial cell recruitment by the transformed cells or by interfering with cell organization in vascular structures. ^[4]

プロトコル(参考用のみ)

キナーゼアッセイ	Enzyme assays
キナーゼアッセイ	In enzyme assays, IC50s for batimastat (BB-94) are determined in vitro against different metalloproteinases.
細胞アッセイ	細胞株	B16-BL6 cells
	濃度	3 nM (MMP-1), 4 nM (MMP-2 & MMP-9), 6 nM (MMP-7) and 20 nM (MMP-3), respectively
	反応時間	22 h
	実験の流れ	Batimastat (BB-94) was dissolved in absolute ethanol, and cells were treated with different concentrations of this compound and were incubated for 22 hr and IC50 was measured.
動物実験	動物モデル	Nude mouse
	投薬量	30mg/ml
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/8050828/
https://pubmed.ncbi.nlm.nih.gov/8610113/
https://pubmed.ncbi.nlm.nih.gov/8062271/

https://pubmed.ncbi.nlm.nih.gov/7535861/

+ 展开

カスタマーフィードバック

: Data from [Data independently produced by , , Nature, 2017, 549(7673):533-537]

: Data from [Data independently produced by , , PLoS One, 2015, 10(5): e0127063]

: Data from [Data independently produced by , , Biomed Rep, 2016, 4(1):73-78. ]

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Extracellularly Detectable Electrochemical Signals of Living Cells Originate from Metabolic Reactions [ Adv Sci (Weinh), 2023, e2207084.]	PubMed: 36737855

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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