Benpyrine racemate

製品コードS3515 バッチS351501

印刷

化学情報

Chemical Structure Synonyms (Rac)-Benpyrine Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C16H16N6O
分子量 308.34 CAS No. 1333714-43-4
Solubility (25°C)* 体外 DMSO 31 mg/mL (100.53 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Benpyrine racemate ((Rac)-Benpyrine) is a potent, highly specific and orally active inhibitor of TNF-α and attenuates TNF-α-induced inflammation, thereby reducing liver and lung injury.
in vitro

Benpyrine directly binds to TNF-α and blocks TNF-α-trigged signaling activation.[1]
in vivo

Benpyrine attenuates TNF-α-induced inflammation in endotoxemic murine model, thereby reducing liver and lung injury, and significantly reliefs the symptoms of collagen-induced arthritis and imiquimod-induced psoriasiform inflammation in mice.[1]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 RAW264.7 macrophages
濃度 5, 10 or 20 μM
反応時間 12 h
実験の流れ

RAW 264.7 cells were grown to 50−70% confluence in a glass chamber, pretreated with DMSO or benpyrine for 12 h, and then stimulated with 10 ng/mL TNF-α for 2 h. Cell treatments were terminated by washing the cells with PBS, followed by fixation with freshly prepared 4% paraformaldehyde in PBS for 20 min. The fixed cells were washed three times with PBS and then permeabilized with 0.25% Triton X-100 in PBS for 10 min. After blocking with 2 mg/mL BSA for 1 h at room temperature, we added the NF-κB/p65 antibody at a 1:1000 dilution at 4 °C overnight, washed three times with PBS, then incubated with a 1:500 dilution of the secondary antibody for 1 h at room temperature in the dark. Finally, 4′,6-diamidino-2-phenyl indole (DAPI) was used to stain the nuclei at 37 °C for 30 min in the dark.
動物実験 動物モデル CIA mice, Imiquimod-Induced Psoriasis-like Skin Lesions mice mode
投薬量 25, 50 mg/kg
投与方法 i.g.

参考

  • https://pubmed.ncbi.nlm.nih.gov/32667202/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Tumor-derived exosomes induce neutrophil infiltration and reprogramming to promote T-cell exhaustion in hepatocellular carcinoma [ Theranostics, 2025, 15(7):2852-2869] PubMed: 40083930

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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