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Synonyms | (Rac)-Benpyrine | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C16H16N6O |
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分子量 | 308.34 | CAS No. | 1333714-43-4 | |
Solubility (25°C)* | 体外 | DMSO | 31 mg/mL (100.53 mM) | |
Water | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Benpyrine racemate ((Rac)-Benpyrine) is a potent, highly specific and orally active inhibitor of TNF-α and attenuates TNF-α-induced inflammation, thereby reducing liver and lung injury. |
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in vitro | Benpyrine directly binds to TNF-α and blocks TNF-α-trigged signaling activation.[1] |
in vivo | Benpyrine attenuates TNF-α-induced inflammation in endotoxemic murine model, thereby reducing liver and lung injury, and significantly reliefs the symptoms of collagen-induced arthritis and imiquimod-induced psoriasiform inflammation in mice.[1] |
細胞アッセイ | 細胞株 | RAW264.7 macrophages |
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濃度 | 5, 10 or 20 μM | |
反応時間 | 12 h | |
実験の流れ | RAW 264.7 cells were grown to 50−70% confluence in a glass chamber, pretreated with DMSO or benpyrine for 12 h, and then stimulated with 10 ng/mL TNF-α for 2 h. Cell treatments were terminated by washing the cells with PBS, followed by fixation with freshly prepared 4% paraformaldehyde in PBS for 20 min. The fixed cells were washed three times with PBS and then permeabilized with 0.25% Triton X-100 in PBS for 10 min. After blocking with 2 mg/mL BSA for 1 h at room temperature, we added the NF-κB/p65 antibody at a 1:1000 dilution at 4 °C overnight, washed three times with PBS, then incubated with a 1:500 dilution of the secondary antibody for 1 h at room temperature in the dark. Finally, 4′,6-diamidino-2-phenyl indole (DAPI) was used to stain the nuclei at 37 °C for 30 min in the dark. |
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動物実験 | 動物モデル | CIA mice, Imiquimod-Induced Psoriasis-like Skin Lesions mice mode |
投薬量 | 25, 50 mg/kg | |
投与方法 | i.g. |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
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