Benzyl isothiocyanate

製品コードS4783 バッチS478301

印刷

化学情報

Chemical Structure Synonyms Benzoylthiocarbimide, Isothiocyanic Acid Benzoyl Ester Storage
(From the date of receipt)		 2 years -20°C liquid
化学式

C8H7NS
分子量 149.21 CAS No. 622-78-6
Solubility (25°C)* 体外 DMSO 29 mg/mL (194.35 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Benzyl isothiocyanate (BITC, Benzoylthiocarbimide, Isothiocyanic Acid Benzoyl Ester) is an isothiocyanate originally found in cruciferous vegetables that exhibits immunomodulatory, anti-parasitic, antibiotic, antioxidative, anti-atherosclerotic, anti-angiogenic, anti-metastatic, anticancer chemotherapeutic, and chemopreventive activities.
in vitro Benzyl isothiocyanate (BITC) is highly effective in suppressing cancer initiation by modulating carcinogen-activating (phase I) and -detoxifying (phase II) enzymes during initiation events of tumor growth. It affects cell-cycle regulation, induction of apoptotic and autophagy events, tumor invasion and metastasis, angiogenesis, and EMT, and it can eliminate CSCs[1]. BITC treatment would cause permanent damage to the cells[2].
in vivo BITC inhibits chemically induced cancer, oncogenic-driven tumor formation, and human tumor xenografts in rodent cancer models. It decreases pulmonary metastasis multiplicity and volume of 4T1 murine mammary carcinoma cells injected into the inguinal mammary fat pads of syngeneic female BALB/c mice. Pharmacokinetic studies suggest that low μmol/L concentrations of BITC are sufficient for tumor inhibitory activities. BITC accumulates rapidly in cancer cells and is conjugated with intracellular thiols, particularly glutathione (GSH) and cysteine[1]. BITC is an inhibitor of B[a]P-induced lung tumorigenesis in A/J mice[3].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Capan-2 cells
濃度 0-40 μmol/L
反応時間 12, 24, 48, or 72 h
実験の流れ The cells are treated with BITC for 12, 24, 48, or 72 h. Effect of BITC on proliferation of Capan-2 or acinar and ductal cells is determined by Sulforhodamine B assay. The plates are read at 570 nm with a Bio Kinetics plate reader.
動物実験 動物モデル Female A/J mice
投薬量 --
投与方法 by gavage

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Benzyl Isothiocyanate-Induced Cytotoxicity via the Inhibition of Autophagy and Lysosomal Function in AGS Cells [ Biomol Ther (Seoul), 2022, 30(4):348-359] PubMed: 35768332
Benzyl Isothiocyanate Induces Apoptosis via Reactive Oxygen Species-Initiated Mitochondrial Dysfunction and DR4 and DR5 Death Receptor Activation in Gastric Adenocarcinoma Cells. [ Biomolecules, 2019, 9(12)] PubMed: 31817791

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人間や獣医の診断であるか治療的な使用のためにでない。

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