β-thujaplicin

製品コードS4771 バッチS477101

印刷

化学情報

Chemical Structure Synonyms Hinokitiol, 4-Isopropyltropolone Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C10H12O2
分子量 164.20 CAS No. 499-44-5
Solubility (25°C)* 体外 DMSO 32 mg/mL (194.88 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 β-Thujaplicin (β-TH, Hinokitiol, 4-Isopropyltropolone) is a toxic tropolone derivative present in the heartwood of western red cedar (Thuja plicata) and is used as a preservative and antimicrobial additive in a number of commercial goods. Hinokitiol is a component of essential oils isolated from Chymacyparis obtusa, reduces Nrf2 expression, and decreases DNMT1 and UHRF1 mRNA and protein expression, with anti-infective, anti-oxidative, and anti-tumor activities.
in vitro In lung cancer cells, hinokitiol inhibits cell proliferation by inducing the p53-independent DNA damage response, autophagy (not apoptosis), S-phase cell cycle arrest, and senescence. Hinokitiol induces autophagy in lung adenocarcinoma cells but not in human lung stromal fibroblasts. It induces cellular senescence in both human lung cancer cells and lung stromal fibroblasts[1]. Treatment with hinokitiol reveals a concentration-dependent inhibition of migration of B16-F10 melanoma cells. It appears to achieve this effect by reducing the expression of MMP-1 and by suppressing the phosphorylation of mitogen- activated protein kinase (MAPK) signaling molecules such as extracellular signal-regulated kinase (ERK) 1/2, p38 MAPK and c-Jun N-terminal kinases (JNK). On the other hand, hinokitiol treatment reverses IκB-α degradation and inhibits the phosphorylation of p65 nuclear factor kappa B (NF-κB) and cJun in B16-F10 cells. In addition, hinokitiol suppresses the translocation of p65 NF-κB from the cytosol to the nucleus, suggesting reduced NF-κB activation[2].
in vivo Hinokitiol reduces tumor growth, potentially through the attenuation of tumorigenicity, and induces DNA damage and autophagy to suppress tumor progression[1]. In vivo study demonstrates that hinokitiol treatment significantly reduces the total number of mouse lung metastatic nodules and improves histological alterations in B16-F10 injected C57BL/6 mice[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 A549 cells
濃度 0.3125-10 µM
反応時間 24, 48, and 72 h
実験の流れ For trypan blue staining, 2×104 cells are cultured in 12-well plates overnight and then incubated with 0.3125-10 µM hinokitiol for 24, 48, and 72 h. At the indicated times, the cells are trypsinized and stained with trypan blue. The viable cells that excluded trypan blue are counted in a counting chamber.
動物実験 動物モデル Six-week old NOD-SCID mice
投薬量 2 or 10 mg/kg
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

The radiosensitizing effect of β-Thujaplicin, a tropolone derivative inducing S-phase cell cycle arrest, in head and neck squamous cell carcinoma-derived cell lines [ Invest New Drugs, 2022, 10.1007/s10637-022-01229-3] PubMed: 35412173

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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