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Synonyms | (+)-Biocytin, Biotinyl-L-lysine | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C16H28N4O4S
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分子量 | 372.48 | CAS No. | 576-19-2 | |
Solubility (25°C)* | 体外 | DMSO | 75 mg/mL (201.35 mM) | |
Water | 75 mg/mL (201.35 mM) | |||
Ethanol | 2 mg/mL (5.36 mM) | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Biocytin ((+)-Biocytin, Biotinyl-L-lysine) is a conjugate of D-biotin and L-lysine. Biocytin is an attractive neuronal tracer. It labels neurons fast over long distances, and it can be used in conjunction with calcium dyes to report on neuronal activity in retrogradely labeled live neurons. |
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in vitro | Biocytin labels type II cells via ion channels activated by [Ca2+]out reduction, probably "CALHM-like" channels, on the basolateral membrane.[3] |
in vivo | Biocytin confers stability to the molecules and increases their applicability as neuronal tract tracer.[2] |
細胞アッセイ | 細胞株 | Taste receptor cells (type II cells) |
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濃度 | 2 mg/ml | |
反応時間 | 5 min | |
実験の流れ | Peeled epithelia are mounted on a recording platform and then are examined under a microscope equipped with a 60× water-immersion objective. Recording electrodes are attached to the basolateral membranes of single TBCs. In these voltage-clamp experiments, voltage-gated currents are amplified and filtered at 10 kHz using a voltage-clamp amplifier. Data are digitized using an A/D converter and are then stored using pCLAMP data acquisition and analysis software on a personal computer. The recording electrodes are filled with an intracellular CsCl solution using a pipette with a resistance of approximately 5 MΩ in physiological saline. To immunohistochemically identify cell types, biocytin (2 mg/ml) is injected through the recording electrodes. |
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動物実験 | 動物モデル | Albino rats (Sprague−Dawley) |
投薬量 | -- | |
投与方法 | ICV |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。