BMH-21

製品コードS7718 バッチS771801

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₁H₂₀N₄O₂

分子量

360.41

CAS No.

896705-16-1

Solubility (25°C)*

体外

DMSO

Insoluble

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution

5%absolute ethyl alcohol 1 95%Corn oil

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

0.250mg/ml (0.69mM)

Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clear absolute ethyl alcohol stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	BMH-21 is a DNA intercalator, which binds ribosomal DNA and inhibits RNA polymerase I (Pol I) transcription.
in vitro	BMH-21 causes proteasome-dependent destruction of RPA194, the large catalytic subunit protein of Pol I holocomplex. ^[1] In U2OS cancer cell line, this compound results in degradation of RPA194 and translocation of NCL with IC50 of 0.05 μM and 0.07 μM, respectively. By causing nucleolar stress, it also shows potent inhibition on cell viability. ^[2]
in vivo	This compound reduces tumor growth in mouse xenografts. ^[1]

製品説明

BMH-21 is a DNA intercalator, which binds ribosomal DNA and inhibits RNA polymerase I (Pol I) transcription.

in vitro

BMH-21 causes proteasome-dependent destruction of RPA194, the large catalytic subunit protein of Pol I holocomplex. ^[1]

In U2OS cancer cell line, this compound results in degradation of RPA194 and translocation of NCL with IC50 of 0.05 μM and 0.07 μM, respectively. By causing nucleolar stress, it also shows potent inhibition on cell viability. ^[2]

in vivo

This compound reduces tumor growth in mouse xenografts. ^[1]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	U2OS osteosarcoma cells
	濃度	~5 μM
	反応時間	48 hours
	実験の流れ	The cells are maintained at 37 °C in a humidified atmosphere containing 5% CO₂. U2OS osteosarcoma cells are cultured in DMEM supplemented with 15% fetal bovine serum. Cells are plated in 96-well plates at a density of 10000 cells/well in triplicate and incubated for 48 h with the compounds. Viability is determined using WST-1 cell proliferation reagent.
動物実験	動物モデル	Athymic NCr nu/nu mice
	投薬量	25 or 50 mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/24434211/
https://pubmed.ncbi.nlm.nih.gov/24847734/
https://pubmed.ncbi.nlm.nih.gov/24434211/

カスタマーフィードバック

: Data from [Data independently produced by , , Cell, 2018, 174(2):338-349]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Hypoxic stress incites HIF1α-driven ribosome biogenesis that can be exploited by targeting RNA Polymerase I [ Nat Commun, 2025, 16(1):8018]	PubMed: 40866413
Colorectal cancer cell line-derived organoid model with stem cell properties captures the regrowing state of residual cancer cells after neoadjuvant chemotherapy [ Cell Death Discov, 2025, 11(1):282]	PubMed: 40537472
DNA-PK participates in pre-rRNA biogenesis independent of DNA double-strand break repair [ Nucleic Acids Res, 2024, gkae316]	PubMed: 38682589
Monoallelically expressed noncoding RNAs form nucleolar territories on NOR-containing chromosomes and regulate rRNA expression [ Elife, 2024, 13e80684]	PubMed: 38240312
Pre-rRNA facilitates the recruitment of RAD51AP1 to DNA double-strand breaks [ J Biol Chem, 2024, 300(3):107115]	PubMed: 38403248
CX‑5461 potentiates imatinib‑induced apoptosis in K562 cells by stimulating KIF1B expression [ Exp Ther Med, 2024, 27(3):107.]	PubMed: 38356673
CX‑5461 potentiates imatinib‑induced apoptosis in K562 cells by stimulating KIF1B expression [ Exp Ther Med, 2024, 27(3):107]	PubMed: 38356673
The ribosomal protein L22 binds the MDM4 pre-mRNA and promotes exon skipping to activate p53 upon nucleolar stress [ bioRxiv, 2024, 10.1101/2023.12.29.573614]	PubMed: none
The ribosomal protein L22 binds the MDM4 pre-mRNA and promotes exon skipping to activate p53 upon nucleolar stress [ bioRxiv, 2024, 10.1101/2023.12.29.573614]	PubMed: none
The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination [ Cell Discov, 2023, 9(1):99]	PubMed: 37789001

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。