BML-190

製品コードS2854 バッチS285401

印刷

化学情報

Chemical Structure Synonyms IMMA Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C23H23ClN2O4
分子量 426.89 CAS No. 2854-32-2
Solubility (25°C)* 体外 DMSO 22 mg/mL (51.53 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 BML-190 (IMMA) is a selective cannabinoid CB2 receptor inverse agonist with Ki of 435 nM, with 50-fold selectivity over CB1 receptor.
in vitro BML-190 has 50-fold selectivity for CB2 receptors over CB1 receptors. In HEK-293 cells stably expressing the human CB2 receptor, this compound potentiates the forskolin-stimulated accumulation of cAMP. It reduces the basal levels of inositol phosphate production in cells expressing the CB2 receptor. 10 μM of this chemical decreases inositol phosphates accumulation by 38%. [1] This compound is an aminoalkylindole. It is found to yield at least 15 metabolic products. [2] It diminishes LPS-induced NO and IL-6 production in a concentration-dependent manner. This compound also inhibits LPS-induced PGE2 production and COX-2 induction. [3]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 293/CB2 cells with [3H]adenine (1 μCi/mL)
濃度 0 μM -100 μM
反応時間 20 hours–24 hours
実験の流れ 293/CBB2 cells are labeled with [3H]adenine (1 μCi/mL) in MEM with 1% FBS for 20 hours-24 hours. Labelled cells are challenged with 50 μM forskolin and appropriate BML-190 for 30 minutes at 37°C and assayed for cAMP accumulation. For IP assays, 2 × 105 293/CB2 cells are transiently transfected with 16z44 and/or pcDNA3 using transfection reagents. Cells are labelled, challenged with this compound and assayed for IP production. Triplicates are performed for each data point and at least three separate trials are done for each ligand.

参考

  • https://pubmed.ncbi.nlm.nih.gov/12586356/
  • https://pubmed.ncbi.nlm.nih.gov/20542112/
  • https://pubmed.ncbi.nlm.nih.gov/11329626/

カスタマーフィードバック

, , University of Alberta, 2014.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Fast-Acting and Receptor-Mediated Regulation of Neuronal Signaling Pathways by Copaiba Essential Oil. [ Int J Mol Sci, 2020, 21(7)] PubMed: 32218156
Novel Therapeutic Approaches for Inflammatory Bowel Disease. [Yahya Muhammad Fiteih , et al. University of Alberta, 2014, Yahya Muhammad Fiteih ]

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。