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Synonyms | Bobcat339 HCl | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C16H13Cl2N3O |
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分子量 | 334.2 | CAS No. | 2436747-44-1 | |
Solubility (25°C)* | 体外 | DMSO | Insoluble | |
Water | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Bobcat339 is a selective cytosine-based TET enzyme inhibitor with IC50 of 33 μM and 73 μM for TET1 and TET2, respectively. Bobcat339 can reduce DNA 5-hydroxymethylcytosine abundance by inhibiting TET enzyme function in living cells and provide support for its utility as a viable pharmacological probe. |
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in vitro | Bobcat339, the TET2 enzyme activity inhibitor, decreases the TET2 enzyme activity and protects cells from neurotoxin damage in SH-SY5Y cells.[2] |
in vivo | Bobcat339 hydrochloride is a potent and selective cytosine-based inhibitor of TET enzyme. |
細胞アッセイ | 細胞株 | SH-SY5Y neuroblastoma cells |
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濃度 | 3 μM | |
反応時間 | 12 h | |
実験の流れ | The CCK-8 assay kit was used to determine cell viability. SH-SY5Y cells are seeded into 96-well plates at a density of 5,000 cells/well with 100 μl of cell medium in each well. Bobcat339 (3 μM) is preincubated with cells for 12 h before MPP+ (2.5 mM) treatment. Then, 10 μl of CCK-8 working solution is added to each well to incubate for about 1 h at 37 C. The wells containing only the culture medium served as blanks. The absorbance value at 450 nm is measured. |
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Ten-eleven translocation-2-mediated macrophage activation promotes liver regeneration [ Cell Commun Signal, 2024, 22(1):95] | PubMed: 38308318 |
Association of DNA methylation/demethylation with the functional outcome of stroke in a hyperinflammatory state [ Neural Regeneration Research, 2024, 19(10):p 2229-2239] | PubMed: none |
TET Family Members Are Integral to Porcine Oocyte Maturation and Parthenogenetic Pre-Implantation Embryogenesis [ Int J Mol Sci, 2023, 24(15)12455] | PubMed: 37569830 |
SIRT1 Deacetylates TET2 and Promotes Its Ubiquitination Degradation to Achieve Neuroprotection Against Parkinson's Disease [ Front Neurol, 2021, 12:652882] | PubMed: 33935952 |
TET2 suppresses nasopharyngeal carcinoma progression by inhibiting glycolysis metabolism [ Cancer Cell Int, 2020, 20:363] | PubMed: 32774157 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。