Bobcat339 hydrochloride

製品コードS6682 バッチS668204

化学情報

	Synonyms	Bobcat339 HCl	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₁₆H₁₃Cl₂N₃O
	分子量	334.2	CAS No.	2436747-44-1
Solubility (25°C)*	体外	DMSO	130 mg/mL (388.98 mM)
		Ethanol	4 mg/mL (11.96 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Bobcat339 is a selective cytosine-based TET enzyme inhibitor with IC50 of 33 μM and 73 μM for TET1 and TET2, respectively. Bobcat339 can reduce DNA 5-hydroxymethylcytosine abundance by inhibiting TET enzyme function in living cells and provide support for its utility as a viable pharmacological probe.
in vitro	Bobcat339, the TET2 enzyme activity inhibitor, decreases the TET2 enzyme activity and protects cells from neurotoxin damage in SH-SY5Y cells.^[2]
in vivo	Bobcat339 hydrochloride is a potent and selective cytosine-based inhibitor of TET enzyme.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	SH-SY5Y neuroblastoma cells
	濃度	3 μM
	反応時間	12 h
	実験の流れ	The CCK-8 assay kit was used to determine cell viability. SH-SY5Y cells are seeded into 96-well plates at a density of 5,000 cells/well with 100 μl of cell medium in each well. Bobcat339 (3 μM) is preincubated with cells for 12 h before MPP+ (2.5 mM) treatment. Then, 10 μl of CCK-8 working solution is added to each well to incubate for about 1 h at 37 C. The wells containing only the culture medium served as blanks. The absorbance value at 450 nm is measured.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Ten-eleven translocation-2-mediated macrophage activation promotes liver regeneration [ Cell Commun Signal, 2024, 22(1):95]	PubMed: 38308318
Association of DNA methylation/demethylation with the functional outcome of stroke in a hyperinflammatory state [ Neural Regeneration Research, 2024, 19(10):p 2229-2239]	PubMed: none
TET Family Members Are Integral to Porcine Oocyte Maturation and Parthenogenetic Pre-Implantation Embryogenesis [ Int J Mol Sci, 2023, 24(15)12455]	PubMed: 37569830
SIRT1 Deacetylates TET2 and Promotes Its Ubiquitination Degradation to Achieve Neuroprotection Against Parkinson's Disease [ Front Neurol, 2021, 12:652882]	PubMed: 33935952
TET2 suppresses nasopharyngeal carcinoma progression by inhibiting glycolysis metabolism [ Cancer Cell Int, 2020, 20:363]	PubMed: 32774157

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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