BRD7552

製品コードS7363 バッチS736302

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₃₃H₃₃N₃O₁₅

分子量

711.63

CAS No.

1137359-47-7

Solubility (25°C)*

体外

DMSO

100 mg/mL (140.52 mM)

Ethanol

100 mg/mL (140.52 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	5mg/ml (7.03mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.71mg/ml (1.00mM)	Taking the 1 mL working solution as an example, add 50 μL of 14.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	BRD7552は、インスリン発現を増加させる転写因子PDX1の誘導剤です。
in vitro	BRD7552 upregulates PDX1 expression in both primary human islets and ductal cells, and induces epigenetic changes in the PDX1 promoter consistent with transcriptional activation. This compound increases PDX1 mRNA levels by 2- to 4-fold, with a maximal effect at 5 μM and the expression of MAFA. It increases PDX1 expression in mouse αTC cells but not βTC cells. Treatment of PANC-1 cells with this chemical causes a dose-dependent increase in insulin mRNA expression.

製品説明

BRD7552は、インスリン発現を増加させる転写因子PDX1の誘導剤です。

in vitro

BRD7552 upregulates PDX1 expression in both primary human islets and ductal cells, and induces epigenetic changes in the PDX1 promoter consistent with transcriptional activation. This compound increases PDX1 mRNA levels by 2- to 4-fold, with a maximal effect at 5 μM and the expression of MAFA. It increases PDX1 expression in mouse αTC cells but not βTC cells. Treatment of PANC-1 cells with this chemical causes a dose-dependent increase in insulin mRNA expression.

プロトコル(参考用のみ)

キナーゼアッセイ	ELISA
キナーゼアッセイ	The Human PDX1 ELISA Kit is used to quantitatively determine PDX1 concentrations in cell lysates. Brieﬂy, 100 μL of standards or samples are added into a 96-well and incubated for 2 h at 37℃. Liquid from each well is removed, and wells are incubated for 1 h with biotinylated anti-body, washed 3 times, and incubated with horseradish peroxidase-avidin. 3,3’,5,5’-Tetramethylbenzidine substrate is added to each well and incubated for 15-30 min at 37℃ followed by stop solution. The optical density of each well is determined within 5 min using a microplate reader set to 450 nm.

参考

https://pubmed.ncbi.nlm.nih.gov/24290880/

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。