Bromopyruvic acid (3-BP)

製品コードS5426 バッチS542602

印刷

化学情報

Synonyms

3-Bromopyruvic acid, 3-Bromopyruvate, Hexokinase II Inhibitor II

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₃H₃BrO₃

分子量

166.96

CAS No.

1113-59-3

Solubility (25°C)*

体外

DMSO

33 mg/mL (197.65 mM)

Water

33 mg/mL (197.65 mM)

Ethanol

33 mg/mL (197.65 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	1.650mg/ml (9.88mM)	Taking the 1 mL working solution as an example, add 50 μL of 33 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	1.650mg/ml (9.88mM)	Taking the 1 mL working solution as an example, add 50 μL of 33 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Bromopyruvic acid (3-BP) is a hexokinase II inhibitor with Ki of 2.4 mM for glycolysis/hexokinase inhibition. It is inhibitor of tumour cell energy metabolism and chemopotentiator of platinum drugs.
in vitro	Bromopyruvic acid, a glycolysis inhibitor, maintains haploidy to a certain extent in both OdG and EG1 haESCs, with no effect on the proliferation or pluripotency of the haESCs.^[2]
in vivo	Bromopyruvic acid reduces the growth of RKO cells with CD36 knockdown at a significantly slower rate in xenograft model.^[3]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Mouse haESC lines AGH-OG-3 and AGH-EG-1
	濃度	12.5 μg/mL
	反応時間	12 days
	実験の流れ	Mouse haESC lines AGH-OG-3 and AGH-EG-1 were cultured in M2iL mediums, which containing knockout DMEM, 15% fetal bovine serum, 1% GlutaMAX, 1% nonessential amino acids, 100 μM β-mercaptoethanol, 100 U Leukemia inhibitory factor, 3 μM CHIR99021, and 1 μM PD0325901. The cells are cultured at 37 ℃ with 5% CO₂ in a humidified environment. 1,25-(OH)₂D3 and 3-BrPA are used at a final concentration of 12.5 μg/mL and 5μM, respectively.
動物実験	動物モデル	4-week-old nude mice injected with RKO cells
	投薬量	5 mg/kg/day
	投与方法	i.p.

参考

https://www.sciencedirect.com/science/article/pii/S1574789108000045
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815943/
https://pubmed.ncbi.nlm.nih.gov/31484922/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Photobiomodulation modulates mitochondrial energy metabolism and ameliorates neurological damage in an APP/PS1 mousmodel of Alzheimer's disease [ Alzheimers Res Ther, 2025, 17(1):72]	PubMed: 40188044
GL-V9 inhibits the activation of AR-AKT-HK2 signaling networks and induces prostate cancer cell apoptosis through mitochondria-mediated mechanism [ iScience, 2024, 27(3):109246]	PubMed: 38439974
Hexokinase 2 nonmetabolic function-mediated phosphorylation of IκBα enhances pancreatic ductal adenocarcinoma progression [ Cancer Sci, 2024, 10.1111/cas.16204]	PubMed: 38801832
METTL14 modulates glycolysis to inhibit colorectal tumorigenesis in p53-wild-type cells [ EMBO Rep, 2023, e56325.]	PubMed: 36794620
3-Bromopyruvate overcomes cetuximab resistance in human colorectal cancer cells by inducing autophagy-dependent ferroptosis [ Cancer Gene Ther, 2023, 10.1038/s41417-023-00648-5]	PubMed: 37558749
ASPP2 suppresses tumour growth and stemness characteristics in HCC by inhibiting Warburg effect via WNT/β-catenin/HK2 axis [ J Cell Mol Med, 2023, 27(5):659-671]	PubMed: 36752127
Microglial hexokinase 2 deficiency increases ATP generation through lipid metabolism leading to β-amyloid clearance [ Nat Metab, 2022, 4(10):1287-1305]	PubMed: 36203054
Integration of glucose and cardiolipin anabolism confers radiation resistance of HCC [ Hepatology, 2022, 75(6):1386-1401]	PubMed: 34580888
Inhibition of abnormally activated HIF-1α-GLUT1/3-glycolysis pathway enhances the sensitivity of hepatocellular carcinoma to 5-caffeoylquinic acid and its derivatives [ Eur J Pharmacol, 2022, S0014-2999(22)00105-4]	PubMed: 35189090
Viral growth factor- and STAT3 signaling-dependent elevation of the TCA cycle intermediate levels during vaccinia virus infection [ PLoS Pathog, 2021, 17(2):e1009303]	PubMed: 33529218

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。