BSO

製品コードS9728 バッチS972801

化学情報

	Synonyms	L-Buthionine sulfoximine, l-BSO	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₈H₁₈N₂O₃S
	分子量	222.31	CAS No.	83730-53-4
Solubility (25°C)*	体外	Water	40 mg/mL (179.92 mM)
		DMSO	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	BSO is a cell-permeable, potent, fast acting and irreversible inhibitor of g-glutamylcysteine synthetase (γ-glutamylcysteine synthetase, γ-GCS) and depletes cellular glutathione levels. The IC50 of BSO on melanoma, breast and ovarian tumor specimens are 1.9 μM, 8.6 μM, and 29 μM, respectively.
in vitro	BSO (L-Buthionine-(S,R)-sulfoximine) synergistically enhanced BCNU activity against melanoma cell lines and human tumors. This compound (50 μM) treatment for 48 hr results in a 95% decrease in ZAZ and M14 melanoma cell line GSH levels, and a 60% decrease in GST enzyme activity. GST-μ protein and mRNA levels are significantly reduced in both cell lines. GST-π expression is unaffected. This chemical enhancement of alkylator action may be related in part to down regulation of GST.^[1]
in vivo	LButhionine-S,R-sulfoximine (L-S,R-BSO) substantially inhibits GSH production at a greater degree and causes a higher toxicity to guinea pigs than mice, implying that mice may have an additional protective mechanism against oxidative stress injury. Indeed, administration of this compound to mice inhibits tissue GSH production while increasing ascorbate levels. This chemical also increases tissue ascorbate levels in mice fed a ascorbate and dehydroascorbatefree diet suggesting activation of ascorbate synthesis, which is further confirmed by increased urinary ascorbate excretion.^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	ZAZ, M14 melanoma cell line
	濃度	50 μM
	反応時間	6 h, 24 h, 48 h, 72 h
	実験の流れ	The effect of BSO treatment on glutathione content (GSH + GSSG) is determined on cells plated in 24-well plates at the same density as in the thymidine incorporation assay. GSH is measured at time zero, and at 6, 24, 48, and 72 hr by a modified Tietze method. GSH standard curves are obtained by dissolving 5 mg GSH in 25 ml 0.01 N HCl and running determinations on serial dilutions. Cell supernatants are prepared by freezethawing cells resuspended to 10⁷ cells per ml in 0.01 M NaP0₄ + 0.005 M EDTA buffer (pH 7.5) three times, and centrifuging for 30 min at 30,000g at 4℃. GSH levels are determined from the rate of change in optical density at 412 nm, 25℃.
動物実験	動物モデル	Swiss-Webster mice, guinea pigs
	投薬量	2.5 mM/kg, 1.25 mM/kg, 0.625 mM/kg
	投与方法	SC

参考

https://pubmed.ncbi.nlm.nih.gov/9263331/
https://pubmed.ncbi.nlm.nih.gov/28115164/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

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A mycobacterial effector promotes ferroptosis-dependent pathogenicity and dissemination [ Nat Commun, 2023, 14(1):1430]	PubMed: 36932056
Preclinical investigations of the efficacy of the glutaminase inhibitor CB-839 alone and in combinations in chronic lymphocytic leukemia [ Front Oncol, 2023, 13:1161254]	PubMed: 37228498
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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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