BSO

製品コードS9728 バッチS972801

印刷

化学情報

Chemical Structure Synonyms L-Buthionine sulfoximine, l-BSO Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C8H18N2O3S
分子量 222.31 CAS No. 83730-53-4
Solubility (25°C)* 体外 Water 40 mg/mL (179.92 mM)
DMSO Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 BSO is a cell-permeable, potent, fast acting and irreversible inhibitor of g-glutamylcysteine synthetase (γ-glutamylcysteine synthetase, γ-GCS) and depletes cellular glutathione levels. The IC50 of BSO on melanoma, breast and ovarian tumor specimens are 1.9 μM, 8.6 μM, and 29 μM, respectively.
in vitro

BSO (L-Buthionine-(S,R)-sulfoximine) synergistically enhanced BCNU activity against melanoma cell lines and human tumors. BSO (50 μM) treatment for 48 hr results in a 95% decrease in ZAZ and M14 melanoma cell line GSH levels, and a 60% decrease in GST enzyme activity. GST-μ protein and mRNA levels are significantly reduced in both cell lines. GST-π expression is unaffected. BSO enhancement of alkylator action may be related in part to down regulation of GST.[1]
in vivo

LButhionine-S,R-sulfoximine (L-S,R-BSO) substantially inhibits GSH production at a greater degree and causes a higher toxicity to guinea pigs than mice, implying that mice may have an additional protective mechanism against oxidative stress injury. Indeed, administration of L-S,R-BSO to mice inhibits tissue GSH production while increasing ascorbate levels. L-S,R-BSO also increases tissue ascorbate levels in mice fed a ascorbate and dehydroascorbatefree diet suggesting activation of ascorbate synthesis, which is further confirmed by increased urinary ascorbate excretion.[2]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 ZAZ, M14 melanoma cell line
濃度 50 μM
反応時間 6 h, 24 h, 48 h, 72 h
実験の流れ

The effect of BSO treatment on glutathione content (GSH + GSSG) is determined on cells plated in 24-well plates at the same density as in the thymidine incorporation assay. GSH is measured at time zero, and at 6, 24, 48, and 72 hr by a modified Tietze method. GSH standard curves are obtained by dissolving 5 mg GSH in 25 ml 0.01 N HCl and running determinations on serial dilutions. Cell supernatants are prepared by freezethawing cells resuspended to 107 cells per ml in 0.01 M NaP04 + 0.005 M EDTA buffer (pH 7.5) three times, and centrifuging for 30 min at 30,000g at 4℃. GSH levels are determined from the rate of change in optical density at 412 nm, 25℃.
動物実験 動物モデル Swiss-Webster mice, guinea pigs
投薬量 2.5 mM/kg, 1.25 mM/kg, 0.625 mM/kg
投与方法 SC

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

SPTBN2 suppresses ferroptosis in NSCLC cells by facilitating SLC7A11 membrane trafficking and localization [ Redox Biol, 2024, 70:103039] PubMed: 38241838
A mycobacterial effector promotes ferroptosis-dependent pathogenicity and dissemination [ Nat Commun, 2023, 14(1):1430] PubMed: 36932056
Preclinical investigations of the efficacy of the glutaminase inhibitor CB-839 alone and in combinations in chronic lymphocytic leukemia [ Front Oncol, 2023, 13:1161254] PubMed: 37228498
ATF4 protects against sorafenib-induced cardiotoxicity by suppressing ferroptosis [ Biomed Pharmacother, 2022, 153:113280] PubMed: 35724508
Intratarget Microdosing for Deep Phenotyping of Multiple Drug Effects in the Live Brain [ Front Bioeng Biotechnol, 2022, 10:855755] PubMed: 35372313
High-throughput in situ perturbation of metabolite levels in the tumor micro-environment reveals favorable metabolic condition for increased fitness of infiltrated T-cells [ Front Cell Dev Biol, 2022, 10:1032360] PubMed: 36619865
Cystine Induced-mTORC2 Activation through Promoting Sin1 Phosphorylation to Suppress Cancer Cell Ferroptosis [ Mol Nutr Food Res, 2022, e2200186] PubMed: 36189894
CDC27-ODC1 Axis Promotes Metastasis, Accelerates Ferroptosis and Predicts Poor Prognosis in Neuroblastoma [ Front Oncol, 2022, 12:774458] PubMed: 35242701
Antioxidant glutathione inhibits inflammation in synovial fibroblasts via PTEN/PI3K/AKT pathway: An in vitro study [ Arch Rheumatol, 2022, 37(2):212-222] PubMed: 36017213
PARP inhibition promotes ferroptosis via repressing SLC7A11 and synergizes with ferroptosis inducers in BRCA-proficient ovarian cancer [ Redox Biol, 2021, S2213-2317(21)00076-8] PubMed: 33722571

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。