Bufalin

製品コードS7821 バッチS782101

印刷

化学情報

Synonyms

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₂₄H₃₄O₄

分子量

386.52

CAS No.

465-21-4

Solubility (25°C)*

体外

DMSO

78 mg/mL (201.8 mM)

Ethanol

40 mg/mL (103.48 mM)

Water

˂1 mg/mL

体内（毎回新しく調製した物を用意してください）

Clear solution

1%DMSO 1 5%Tween80 2 3%PEG300 3 91%ddH2O

0.4mg/ml

Taking the 1 mL working solution as an example, add 10 μL 40 mg/ml clarified DMSO stock solution to 50 μL Tween80, mix evenly to clarify it; add 30 μL PEG300 to the above system, mix evenly to clarify it; then continue to add 910 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Bufalin (BF) is a potent inhibitor of steroid receptor coactivator SRC-3, SRC-1 and Na+/K+-ATPase. Bufalin binds to the subunit α1, α2 and α3 of Na+/K+-ATPase with Kd of 42.5 nM, 45 nM and 40 nM, respectively. Bufalin is a major digoxin-like immunoreactive component isolated from the Chinese medicine Chan Su with anti-cancer activity.
in vitro	Bufalin strongly promotes SRC-3 protein degradation and binds directly to the coactivator. Bufalin is able to block cancer cell growth at nanomolar concentrations.^[2]
in vivo	When incorporated into a nanoparticle delivery system, bufalin is able to reduce tumor growth in a mouse xenograft model of breast cancer. Bufalin sensitizes cancer cells to other targeted chemotherapeutic agents.^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HeLa, MCF-7, A549 cells
	濃度	2 nM, 5 nM, 7.5 nM
	反応時間	24 h
	実験の流れ	Luciferase assays are performed in HeLa cells transiently transfected with the reporter vector pG5-LUC in combination with expression vectors for pBIND, pBIND SRC-1, SRC-2 or SRC-3 prior to incubation with bufalin of different doses (0, 2, 5 and 7.5 nM) for 24 hours.
動物実験	動物モデル	6-7 week old SCID mice injected with MDA-MB-231-LM3.3 cells
	投薬量	1.5 mg/kg
	投与方法	IV

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

UBA3 promotes the occurrence and metastasis of intrahepatic cholangiocarcinoma through MAPK signaling pathway [ Acta Biochim Biophys Sin (Shanghai), 2024, 56(2):199-209]	PubMed: 38298057
Cell membrane-camouflaged bufalin targets NOD2 and overcomes multidrug resistance in pancreatic cancer [ Drug Resist Updat, 2023, 71:101005]	PubMed: 37647746
Bufalin targeting CAMKK2 inhibits the occurrence and development of intrahepatic cholangiocarcinoma through Wnt/β-catenin signal pathway [ J Transl Med, 2023, 21(1):900]	PubMed: 38082327
USP24-dependent stabilization of Runx2 recruits a p300/NCOA3 complex to transactivate ADAMTS genes and promote degeneration of intervertebral disc in chronic inflammation mice [ Biol Direct, 2023, 18(1):37]	PubMed: 37415159
Alisol B 23-Acetate Increases the Antitumor Effect of Bufalin on Liver Cancer through Inactivating Wnt/β-Catenin Axis [ Comput Math Methods Med, 2022, 2022:6249534]	PubMed: 35572840
Elevated Sodium Pump α3 Subunit Expression Promotes Colorectal Liver Metastasis via the p53-PTEN/IGFBP3-AKT-mTOR Axis [ Front Oncol, 2021, 11:743824]	PubMed: 34868940
Reversal of Infected Host Gene Expression Identifies Repurposed Drug Candidates for COVID-19 [ bioRxiv, 2020, 2020/9/20.4.7.30734]	PubMed: 32511305

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。