Bufexamac

製品コードS3023 バッチS302301

印刷

化学情報

Synonyms

Bufexamic acid

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₁₂H₁₇NO₃

分子量

223.27

CAS No.

2438-72-4

Solubility (25°C)*

体外

DMSO

45 mg/mL (201.54 mM)

Ethanol

3 mg/mL (13.43 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	2.25mg/ml (10.08mM)	Taking the 1 mL working solution as an example, add 50 μL of 45 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.32mg/ml (1.43mM)	Taking the 1 mL working solution as an example, add 50 μL of 6.4 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Bufexamac(Bufexamic acid) is a COX inhibitor for IFN-α release with EC50 of 8.9 μM.
in vitro	Bufexamac is a specific inhibitor of class IIB histone deacetylases (HDAC6 and HDAC10). Treatment of peripheral blood mononuclear cells with this compound inhibits the secretion of IFN-α. ^[1] This chemical is a frequent and relevant contact sensitizer. It is a non-steroidal anti-inflammatory drug. ^[2]
特徴	A specific inhibitor of class IIB histone deacetylases.

プロトコル(参考用のみ)

キナーゼアッセイ	Enzymatic deacetylation assays
キナーゼアッセイ	The enzyme activity of purified recombinant HDAC1, HDAC2, HDAC3-NCoR and HDAC6 (1 μg of protein per well) is measured using the Active Motif HDAC Assay Kit in 96-well format following the manufacturer

参考

https://pubmed.ncbi.nlm.nih.gov/21258344/
https://pubmed.ncbi.nlm.nih.gov/9165205/

カスタマーフィードバック

: , , Nat Biotechnol, 2015, 33(4):415-23.

: Data from [Data independently produced by , , Oncotarget, 2016, 7(37):59388-59401]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

In-Cell Testing of Zinc-Dependent Histone Deacetylase Inhibitors in the Presence of Class-Selective Fluorogenic Substrates: Potential and Limitations of the Method [ Biomedicines, 2024, 12(6)1203]	PubMed: 38927410
Pre-Senescence Induction in Hepatoma Cells Favors Hepatitis C Virus Replication and Can Be Used in Exploring Antiviral Potential of Histone Deacetylase Inhibitors [ Int J Mol Sci, 2021, 22(9)4559]	PubMed: 33925399
HDAC6 Is Involved in the Histone Deacetylation of In Vitro Maturation Oocytes and the Reprogramming of Nuclear Transplantation in Pig [ Reprod Sci, 2021, 10.1007/s43032-021-00533-2]	PubMed: 33725312
Absence of autophagy promotes apoptosis by modulating the ROS-dependent RLR signaling pathway in classical swine fever virus-infected cells. [ Autophagy, 2016, 12(10):1738-1758]	PubMed: 27463126
HDAC10 promotes lung cancer proliferation via AKT phosphorylation. [Yang Y, et al. Oncotarget, 2016, 7(37):59388-59401]	PubMed: 27449083
Acetylation site specificities of lysine deacetylase inhibitors in human cells. [Scholz C, et al. Nat Biotechnol, 2015, 10.1038/nbt.3130]	PubMed: 25751058

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。