Busulfan

製品コードS1692 バッチS169203

印刷

化学情報

Chemical Structure Synonyms NSC-750 Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C6H14O6S2
分子量 246.3 CAS No. 55-98-1
Solubility (25°C)* 体外 DMSO 49 mg/mL (198.94 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Busulfanは、細胞周期非特異的なアルキル化抗悪性腫瘍薬です。DNAとDNA、およびDNAとタンパク質の架橋によりDNA損傷を引き起こします。BusulfanはThioredoxin Reductase活性を阻害します。また、Apoptosis relatedも誘導します。これは免疫抑制性および骨髄破壊性の化学療法剤です。Busulfan (NSC-750)は貧血の動物モデルを誘導するために使用できます。
in vitro

Busulfan inhibits the cobblestone area-forming cell frequency but fails to cause a significant increase in apoptosis in hematopoietic stem cell alike cells and progenitors. This compound inhibits the hematopoietic function of HSC alike cells and progenitors via an apoptosis-independent mechanism. It induces bone marrow hematopoietic cell senescence associated with an increased expression of p16Ink4a and p19Arf in a time-dependent manner. This chemical, an alkylating agent that causes DNA damage by cross-linking DNAs and DNA and proteins, induces senescence in normal human diploid WI38 fibroblasts through the extracellular signal-regulated kinase (Erk) and p38 mitogen-activated protein kinase (p38 MAPK) cascade independent of the p53-DNA damage pathway. It induces a transient reduction in GSH but a continuous increase in ROS production. This compound-induced hypophosphorylation of Rb prevents apoptosis of spermatogonial stem cells by inhibiting PCNA expression in testicular cells.

in vivo

Busulfan-treated mice exhibit a marked increase in apoptosis and a decrease in testis weight. This compound is administered at the rate of 40 mg/kg body weight to induce a maximal number of apoptotic cells while minimizing the number of necrotic cells. Its conditioning and irradiation results in comparable sensitivity of HSC detection as evaluated by limiting dilution analysis in NOD/SCID mice. This chemical-transplanted mice has slow and incomplete lymphoid engraftment. It (20 mg/kg to 100 mg/kg) provides dose-dependent congenic lymphoid reconstitution in mice.

密度 1.4 g/mL

プロトコル(参考用のみ)

参考

  • https://pubmed.ncbi.nlm.nih.gov/14500376/
  • https://pubmed.ncbi.nlm.nih.gov/17512465/
  • https://pubmed.ncbi.nlm.nih.gov/15388331/
  • https://pubmed.ncbi.nlm.nih.gov/17018389/
  • https://pubmed.ncbi.nlm.nih.gov/17018389/
  • https://pubmed.ncbi.nlm.nih.gov/22286157/

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。