受注:045-509-1970 |
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Synonyms | Butyl parahydroxybenzoate, Butyl paraben, Butyl 4-hydroxybenzoate | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C11H14O3 |
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分子量 | 194.23 | CAS No. | 94-26-8 | |
Solubility (25°C)* | 体外 | DMSO | 38 mg/mL (195.64 mM) | |
Water | 38 mg/mL (195.64 mM) | |||
Ethanol | 38 mg/mL (195.64 mM) | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Butylparaben (Butyl parahydroxybenzoate, Butyl 4-hydroxybenzoate) is a chemical compound commonly used as an antifungal preservative in cosmetic products. |
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in vitro | After butylparaben treatment, vimentin filaments in Sertolicells are disrupted. Sertoli cell cytoskeleton responds to butylparaben by exhibiting a marked collapse in vimentin filaments distribution[1]. |
in vivo | Butylparaben induces collapse of Sertoli cell vimentin filaments in vivo[1]. Exposure of a postweaning mammal to butylparaben had an adverse effect on the secretion of testosterone and in the functions of the male reproductive system[2]. |
細胞アッセイ | 細胞株 | Primary Sertoli cell |
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濃度 | 0, 1, 100 and 1000 nmol/ml | |
反応時間 | 6, 24h | |
実験の流れ | Mixed cultures of Sertoli and spermatogenic cells are prepared from three-week-old SD rats. Single cells are obtained by gravity sedimentation and use of 50% trypsin-EDTA. Cells are finally suspended at a concentration of 1 × 108cell/ml and maintained for 24 h in Dulbecco’s modified Eagle medium supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, and 10% fetal bovineserum. Thereafter, cells are maintained in serum-freemedium, which is changed every 24 h. At 72 h, spermatogenic cells are removed by treatment with hypotonic Tris buffer (20 mMTris–HCl, pH 7.4) for 5 min. The Sertoli cell-enriched cultures obtained are then maintained in serum-free medium for 24 h before being reseeded onto chamber slides. The samples are cultured for a further 5 days. Subsequently, they are exposed to 0, 1, 100 and 1000 nmol/ml butylparaben for 6 and 24 h. Some slides are stained with 0.5% toluidine blue for light microscopic observations, whereas the rest are used for vimentin immuno-histochemical analysis. |
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動物実験 | 動物モデル | Male Sprague–Dawley rats |
投薬量 | 1000 mg/kg | |
投与方法 | oral administration |
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Butyl paraben promotes apoptosis in human trophoblast cells through increased oxidative stress-induced endoplasmic reticulum stress [ Environ Toxicol, 2018, 33(4):436-445] | PubMed: 29319206 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。