C75

製品コードS9819 バッチS981901

印刷

化学情報

Chemical Structure Synonyms Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C14H22O4
分子量 254.32 CAS No. 218137-86-1
Solubility (25°C)* 体外 DMSO 51 mg/mL (200.53 mM)
Ethanol 51 mg/mL (200.53 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
3.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 60 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO Corn oil
3.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 60 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 C75 (4-Methylene-2-octyl-5-oxotetrahydrofuran-3-carboxylic acid) , a more stable synthetic mimic of cerulenin, inhibits fatty-acid synthase (FAS/FASN) with an IC50 of 15.53 μM and has potential therapeutic effects in several cancer models. C75 is also a potent CPT1A activator.
in vitro

C75, the competitive irreversible fatty acid synthase (FASN) inhibitor, possesses radiosensitizing activity in prostate cancer cell lines that is affected by the availability of fatty acids, also increases expression of fatty acid transporter proteins FATP1 and CD36.[3]
in vivo

C75, an alpha-methylene-gamma-butyrolactone, reduces adipose tissue and fatty liver in treated DIO mice, despite high levels of malonyl-CoA.[4]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 PC3, UVW and LNCaP cell lines, SK-N-BE(2c) and MCF7 cells
濃度 35 µM
反応時間 24 h
実験の流れ

Cells are seeded in 25 cm2 flasks at 105 cells/flask. When cultures are in exponential growth phase, medium is removed and replaced with fresh medium (including 1% or 10% serum) containing C75 (35 mM) and simultaneous administration of x-rays (2 Gy). Cells are then incubated for 24 h at 37℃ in 5% CO2. After treatment, cells are counted and seeded for clonogenic survival assay.  
動物実験 動物モデル Diet-induced obese mice model
投薬量 20, 15, 10, 15, 15 mg/kg
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Restoration of CPT1A-mediated fatty acid oxidation in mesothelial cells protects against peritoneal fibrosis [ Theranostics, 2023, 13(13):4482-4496] PubMed: 37649600
Restoration of CPT1A-mediated fatty acid oxidation in mesothelial cells protects against peritoneal fibrosis [ Theranostics, 2023, 10.7150/thno.84921] PubMed: 37649600
Enterovirus A71 utilizes host cell lipid β-oxidation to promote its replication [ Front Microbiol, 2022, 13:961942] PubMed: 36246276

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。