C-DIM12

製品コードS8097 バッチS809701

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C23H17ClN2
分子量 356.85 CAS No. 178946-89-9
Solubility (25°C)* 体外 DMSO 71 mg/mL (198.96 mM)
Ethanol 71 mg/mL (198.96 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 C-DIM12 is a Nurr1 activator that stimulates Nurr1 mediated apoptosis axis in bladder cancer cells and tumors and inhibits NF-κB–dependent gene expression in glial cells.
in vitro C-DIM12 induces Nurr1 and DA gene expression in cell lines and primary neurons[3]. This compound suppresses astrocyte inflammatory signaling in vitro. It inhibits lipopolysaccharide (LPS)–induced expression of NF-κB-regulated genes in BV-2 microglia including nitric oxide synthase (NOS2), interleukin-6 (IL-6), and chemokine (C-C motif) ligand 2 (CCL2), and the effects were attenuated by Nurr1-RNA interference. Additionally, this chemical decreased NF-κB activation in NF-κB–GFP (green fluorescent protein) reporter cells and enhanced nuclear translocation of Nurr1 primary microglia. It decreases lipopolysaccharide-induced p65 binding to the NOS2 promoter and concurrently enhanced binding of Nurr1 to the p65-binding site. This compound also stabilized binding of the Corepressor for Repressor Element 1 Silencing Transcription Factor (CoREST) and the Nuclear Receptor Corepressor 2 (NCOR2)[2].
in vivo C-DIM12 has the neuroprotective activity in MPTPp-treated mice[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HEK293 cells
濃度 100 μM
反応時間 24 h
実験の流れ NF-κB–GFP HEK cells are exposed to 30 ng/ml of TNFα in the presence of 100 μM C-DIM12 for up to 24 hours. This compound is efficient at blocking NF-κB–GFP expression in the NF-κB–GFP HEK cells after TNFα treatment, displaying a statistically significant reduction in total GFP fluorescence per cell.
動物実験 動物モデル Transgenic NF-κB/EGFP reporter mice (C57Bl/6 background)
投薬量 50 mg/kg
投与方法 oral administration

参考

  • https://pubmed.ncbi.nlm.nih.gov/25406165/
  • https://pubmed.ncbi.nlm.nih.gov/25858541/
  • https://pubmed.ncbi.nlm.nih.gov/26383113/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

α-Synuclein Negatively Regulates Nurr1 Expression Through NF-κB-Related Mechanism [ Front Mol Neurosci, 2020, 23724] PubMed: 32477062
miR-381-3p knockdown improves intestinal epithelial proliferation and barrier function after intestinal ischemia/reperfusion injury by targeting nurr1. [ Cell Death Dis, 2018, 9(3):411] PubMed: 29540663

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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