C-DIM12

製品コードS8097 バッチS809702

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₂₃H₁₇ClN₂
	分子量	356.85	CAS No.	178946-89-9
Solubility (25°C)*	体外	DMSO	71 mg/mL (198.96 mM)
		Ethanol	71 mg/mL (198.96 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	C-DIM12 is a Nurr1 activator that stimulates Nurr1 mediated apoptosis axis in bladder cancer cells and tumors and inhibits NF-κB–dependent gene expression in glial cells.
in vitro	C-DIM12 induces Nurr1 and DA gene expression in cell lines and primary neurons^[3]. C-DIM12 suppresses astrocyte inflammatory signaling in vitro. C-DIM12 inhibits lipopolysaccharide (LPS)–induced expression of NF-κB-regulated genes in BV-2 microglia including nitric oxide synthase (NOS2), interleukin-6 (IL-6), and chemokine (C-C motif) ligand 2 (CCL2), and the effects were attenuated by Nurr1-RNA interference. Additionally, C-DIM12 decreased NF-κB activation in NF-κB–GFP (green fluorescent protein) reporter cells and enhanced nuclear translocation of Nurr1 primary microglia. C-DIM12 decreases lipopolysaccharide-induced p65 binding to the NOS2 promoter and concurrently enhanced binding of Nurr1 to the p65-binding site. C-DIM12 also stabilized binding of the Corepressor for Repressor Element 1 Silencing Transcription Factor (CoREST) and the Nuclear Receptor Corepressor 2 (NCOR2)^[2].
in vivo	C-DIM12 has the neuroprotective activity in MPTPp-treated mice^[2].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HEK293 cells
	濃度	100 μM
	反応時間	24 h
	実験の流れ	NF-κB–GFP HEK cells are exposed to 30 ng/ml of TNFα in the presence of 100 μM C-DIM12 for up to 24 hours. C-DIM12 is efficient at blocking NF-κB–GFP expression in the NF-κB–GFP HEK cells after TNFα treatment, displaying a statistically significant reduction in total GFP fluorescence per cell.
動物実験	動物モデル	Transgenic NF-κB/EGFP reporter mice (C57Bl/6 background)
	投薬量	50 mg/kg
	投与方法	oral administration

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

α-Synuclein Negatively Regulates Nurr1 Expression Through NF-κB-Related Mechanism [ Front Mol Neurosci, 2020, 23724]	PubMed: 32477062
miR-381-3p knockdown improves intestinal epithelial proliferation and barrier function after intestinal ischemia/reperfusion injury by targeting nurr1. [ Cell Death Dis, 2018, 9(3):411]	PubMed: 29540663

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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