Datasheet

生物学的記述

Specificity	c-Kit Antibody [D16J9] detects endogenous levels of total c-Kit protein.
Background	c‑Kit is a type III receptor tyrosine kinase that belongs to the same subfamily as the PDGF, CSF‑1, and FLT3 receptors and is expressed in hematopoietic stem cells, mast cells, melanocytes, germ cells, interstitial cells of Cajal, and several neuronal populations. Upon binding of its ligand stem cell factor (SCF), c‑Kit undergoes dimerization and autophosphorylation on multiple cytoplasmic tyrosine residues, which serve as docking sites for SH2‑containing adaptor and effector proteins. Phosphorylated c‑Kit recruits and activates the p85 subunit of PI3‑kinase, PLCγ, SHP2, CrkL, and other signaling components, thereby initiating downstream PI3K/AKT, Ras/MAPK, JAK/STAT, and Src‑family kinase pathways that regulate cell survival, proliferation, migration, and differentiation. A specific tyrosine residue in the kinase insert region, Tyr719 (Y719), is essential for binding of the p85 subunit of PI3‑kinase and for c‑Kit‑associated PI3‑kinase activity, positioning this site as a key node for lipid‑mediated signaling. Activating mutations in the c‑Kit gene, particularly in exons encoding the juxtamembrane and kinase domains, are detected in a high proportion of gastrointestinal stromal tumors (GISTs) and in systemic mastocytosis, where constitutive receptor activity drives aberrant growth and survival of tumor cells. In contrast, loss‑of‑function or hypomorphic c‑Kit alleles are associated with developmental defects affecting hematopoiesis, melanogenesis, and gametogenesis, underscoring the receptor’s central role in tissue homeostasis.

Specificity

c-Kit Antibody [D16J9] detects endogenous levels of total c-Kit protein.

Background

c‑Kit is a type III receptor tyrosine kinase that belongs to the same subfamily as the PDGF, CSF‑1, and FLT3 receptors and is expressed in hematopoietic stem cells, mast cells, melanocytes, germ cells, interstitial cells of Cajal, and several neuronal populations. Upon binding of its ligand stem cell factor (SCF), c‑Kit undergoes dimerization and autophosphorylation on multiple cytoplasmic tyrosine residues, which serve as docking sites for SH2‑containing adaptor and effector proteins. Phosphorylated c‑Kit recruits and activates the p85 subunit of PI3‑kinase, PLCγ, SHP2, CrkL, and other signaling components, thereby initiating downstream PI3K/AKT, Ras/MAPK, JAK/STAT, and Src‑family kinase pathways that regulate cell survival, proliferation, migration, and differentiation. A specific tyrosine residue in the kinase insert region, Tyr719 (Y719), is essential for binding of the p85 subunit of PI3‑kinase and for c‑Kit‑associated PI3‑kinase activity, positioning this site as a key node for lipid‑mediated signaling. Activating mutations in the c‑Kit gene, particularly in exons encoding the juxtamembrane and kinase domains, are detected in a high proportion of gastrointestinal stromal tumors (GISTs) and in systemic mastocytosis, where constitutive receptor activity drives aberrant growth and survival of tumor cells. In contrast, loss‑of‑function or hypomorphic c‑Kit alleles are associated with developmental defects affecting hematopoiesis, melanogenesis, and gametogenesis, underscoring the receptor’s central role in tissue homeostasis.

使用情報

Application

WB, IP, IF

Dilution

WB	IP	IF
1:1000	1:50	1:400

Reactivity

Human, Mouse

Source

Rabbit Monoclonal Antibody

MW

120, 145 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/23678293/
https://pubmed.ncbi.nlm.nih.gov/23073628/

Application Data

WB

Validated by Selleck

Lane 1: NCI-H526