Capsaicin(Vanilloid)

製品コードS1990 バッチS199002

印刷

化学情報

Chemical Structure Synonyms Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C18H27NO3
分子量 305.41 CAS No. 404-86-4
Solubility (25°C)* 体外 DMSO 61 mg/mL (199.73 mM)
Ethanol 61 mg/mL (199.73 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Capsaicin(Vanilloid) is an active component of chili peppers, which are plants belonging to the genus Capsicum.Capsaici is a TRPV1 agonist with an EC50 of 0.29 μM in HEK293 cells.Capsaicin is a mixture of Capsaicin and Dihydrocapsaicin.
in vitro

Capsaicin, a TRPV-1 agonist, dose-dependently inhibits the proliferation of the HCC cell lines PLC/PRF/7, HuH7 and HepG2, which is not effected by TRPV1 channel blockers. Combining capsaicin and sorafenib significantly enhances the suppression on cell proliferation, achieving a high-level synergistic effect and promoting HCC cell apoptosis through the enhancement of p-ERK and reduction of p-STAT3 signaling.[1]
in vivo

Combining capsaicin and sorafenib enhances inhibition of xenograft tumor growth in nude mice, with no apparent gross toxicity.[1]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HCC cells PLC/PRF/5, HuH7, and HepG2
濃度 50, 100, 150, 200 and 250 μM
反応時間 72 h
実験の流れ

Cells (5×103 per well) seeded in 96-well plates are exposed to capsaicin, sorafenib, or their combination for 72 h. Then, the medium is removed and the wells are washed with phosphate-buffered saline (PBS). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is performed by adding 20 μL of MTT to the cells for 4 h. Light absorbance is measured.
動物実験 動物モデル nude mice of PLC/PRF/5 tumor xenograft model
投薬量 200 μM
投与方法 i.t.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Phytoformulation with hydroxycitric acid and capsaicin protects against high-fat-diet-induced obesity cardiomyopathy by reducing cardiac lipid deposition and ameliorating inflammation and apoptosis in the heart [ J Tradit Complement Med, 2024, 14(2):162-172] PubMed: 38481548
Effects of Oncolytic Vaccinia Viruses Harboring Different Marine Lectins on Hepatocellular Carcinoma Cells [ Int J Mol Sci, 2023, 24(4)3823] PubMed: 36835232
Interaction between TRPML1 and p62 in Regulating Autophagosome-Lysosome Fusion and Impeding Neuroaxonal Dystrophy in Alzheimer's Disease [ Oxid Med Cell Longev, 2022, 2022:8096009] PubMed: 35116093
Oncolytic Vaccinia Virus Harboring Aphrocallistes vastus Lectin Inhibits the Growth of Hepatocellular Carcinoma Cells [ Mar Drugs, 2022, 20(6)378] PubMed: 35736181
Electroacupuncture Pretreatment Attenuates Cerebral Ischemia-Reperfusion Injury in Rats Through Transient Receptor Potential Vanilloid 1-Mediated Anti-apoptosis via Inhibiting NF-κB Signaling Pathway [ Neuroscience, 2021, S0306-4522(21)00641-2] PubMed: 34929338
Paeoniflorin relieves LPS-induced inflammatory pain in mice by inhibiting NLRP3 inflammasome activation via transient receptor potential vanilloid 1. [ J Leukoc Biol, 2020, 10.1002/JLB.3MA0220-355R] PubMed: 32083340
Electroacupuncture Pretreatment Elicits Neuroprotection Against Cerebral Ischemia-Reperfusion Injury in Rats Associated with Transient Receptor Potential Vanilloid 1-Mediated Anti-Oxidant Stress and Anti-Inflammation [ Inflammation, 2019, 10.1007/s10753-019-01040-y] PubMed: 31190106
Capsaicin enhances the antitumor activity of sorafenib in hepatocellular carcinoma cells and mouse xenograft tumors through increased ERK signaling [ Acta Pharmacol Sin, 2018, 39(3):438-448] PubMed: 29188798
Capsaicin and sorafenib combination treatment exerts synergistic anti‑hepatocellular carcinoma activity by suppressing EGFR and PI3K/Akt/mTOR signaling [ Oncol Rep, 2018, 40(6):3235-3248] PubMed: 30272354
Nerve growth factor rescues diabetic mice heart after ischemia/reperfusion injury via up-regulation of the TRPV1 receptor. [ J Diabetes Complications, 2015, 29(3):323-8] PubMed: 25650182

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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