Capsaicin(Vanilloid)

製品コードS1990 バッチS199003

印刷

化学情報

Chemical Structure Synonyms Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C18H27NO3
分子量 305.41 CAS No. 404-86-4
Solubility (25°C)* 体外 DMSO 54 mg/mL (176.81 mM)
Ethanol 54 mg/mL (176.81 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO Corn oil

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 2.700mg/ml (8.84mM) Taking the 1 mL working solution as an example, add 50 μL of 54 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Capsaicin(Vanilloid) is an active component of chili peppers, which are plants belonging to the genus Capsicum.Capsaici is a TRPV1 agonist with an EC50 of 0.29 μM in HEK293 cells.Capsaicin is a mixture of Capsaicin and Dihydrocapsaicin.
in vitro

A TRPV-1 agonist, capsaicin (Vanilloid) dose-dependently inhibits the proliferation of the HCC cell lines PLC/PRF/7, HuH7 and HepG2, which is not effected by TRPV1 channel blockers. Combining it and sorafenib significantly enhances the suppression on cell proliferation, achieving a high-level synergistic effect and promoting HCC cell apoptosis through the enhancement of p-ERK and reduction of p-STAT3 signaling.[1]
in vivo

Combining capsaicin and sorafenib enhances inhibition of xenograft tumor growth in nude mice, with no apparent gross toxicity.[1]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HCC cells PLC/PRF/5, HuH7, and HepG2
濃度 50, 100, 150, 200 and 250 μM
反応時間 72 h
実験の流れ

Cells (5×103 per well) seeded in 96-well plates are exposed to capsaicin (vanilloid), sorafenib, or their combination for 72 h. Then, the medium is removed and the wells are washed with phosphate-buffered saline (PBS). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is performed by adding 20 μL of MTT to the cells for 4 h. Light absorbance is measured.
動物実験 動物モデル nude mice of PLC/PRF/5 tumor xenograft model
投薬量 200 μM
投与方法 i.t.

参考

  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843835/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Keratin 6A promotes skin inflammation through JAK1-STAT3 activation in keratinocytes [ J Biomed Sci, 2025, 32(1):47] PubMed: 40346694
Plant Secondary Metabolites as Modulators of Mitochondrial Health: An Overview of Their Anti-Oxidant, Anti-Apoptotic, and Mitophagic Mechanisms [ Int J Mol Sci, 2025, 26(1)380] PubMed: 39796234
CB1 Receptor Agonist ACEA Resists ER Stress-Mediated Apoptosis via CB1R-Independent Mechanism [ Biol Pharm Bull, 2025, 48(6):769-781] PubMed: 40467480
The sensory nerve regulates stem cell homeostasis through Wnt5a signaling [ iScience, 2024, 27(10):111035] PubMed: 39635121
CGRP Released by Corneal Sensory Nerve Maintains Tear Secretion of the Lacrimal Gland [ Invest Ophthalmol Vis Sci, 2024, 65(4):30] PubMed: 38635244
Phytoformulation with hydroxycitric acid and capsaicin protects against high-fat-diet-induced obesity cardiomyopathy by reducing cardiac lipid deposition and ameliorating inflammation and apoptosis in the heart [ J Tradit Complement Med, 2024, 14(2):162-172] PubMed: 38481548
Heat promotes melanogenesis by increasing the paracrine effects in keratinocytes via the TRPV3/Ca2+/Hh signaling pathway [ iScience, 2023, 26(5):106749] PubMed: 37216091
Effects of Oncolytic Vaccinia Viruses Harboring Different Marine Lectins on Hepatocellular Carcinoma Cells [ Int J Mol Sci, 2023, 24(4)3823] PubMed: 36835232
Interaction between TRPML1 and p62 in Regulating Autophagosome-Lysosome Fusion and Impeding Neuroaxonal Dystrophy in Alzheimer's Disease [ Oxid Med Cell Longev, 2022, 2022:8096009] PubMed: 35116093
Oncolytic Vaccinia Virus Harboring Aphrocallistes vastus Lectin Inhibits the Growth of Hepatocellular Carcinoma Cells [ Mar Drugs, 2022, 20(6)378] PubMed: 35736181

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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