Carbamoylcholine chloride (Carbachol)

製品コードS4359 バッチS435901

印刷

化学情報

Synonyms

Carbamylcholine chloride, Carbastat, Miostat

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₆H₁₅N₂O₂.Cl

分子量

182.65

CAS No.

51-83-2

Solubility (25°C)*

体外

Water

36 mg/mL (197.09 mM)

Ethanol (warmed with 50ºC water bath)

12 mg/mL (65.69 mM)

DMSO (warmed with 50ºC water bath)

5 mg/mL (27.37 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Carbamoylcholine chloride (Carbachol, Carbamylcholine chloride, Carbastat, Miostat) is a cholinergic agonist that mimics the effect of acetylcholine on both the muscarinic and nicotinic receptors. This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.
in vitro	Carbamoylcholine chloride (Carbachol) causes an immediate increase in short circuit current (Isc) that peaked within 5 min and declined rapidly thereafter in T84 cell monolayers with IC50 of 10 mM, although a small increase in Isc persisted for approximately 30 min. It increases basolateral K+ efflux by activating two different K+ transport pathways on the basolateral membrane in T84 cell monolayers. ^[1] This compound (100 μM) stimulates a time-dependent increase in tyrosine phosphorylation of a range of proteins in T84 cells. It also stimulates an increase in both phosphorylation and activity of the extracellular signal-regulated kinase (ERK) isoforms of mitogen-activated protein kinase in T84 cells. Additionally, it stimulates tyrosine phosphorylation of the EGF receptor (EGFr) and increases co-immunoprecipitation of the adapter proteins, Shc and Grb2, with the EGFr in T84 cells. ^[2] At a 3.3 nmol dose, it produces a transient increase in NGF mRNA levels present 4-8 hours in hippocampus of rats. It also causes transient decreases in NT-3 mRNA levels in dentate gyrus and CA2 with pilocarpine showing a similar trend in rats. ^[3]
in vivo	Infused specifically into the posterior oral pontine reticular nucleus (PnO) of the rat, Carbamoylcholine chloride (Carbachol) (1–10 ng) induces rapid eye movement sleep. At 500 ng, it does not affect REM sleep but enhances wakefulness. ^[4]

プロトコル(参考用のみ)

参考

https://pubmed.ncbi.nlm.nih.gov/3003156/
https://pubmed.ncbi.nlm.nih.gov/9765228/
https://pubmed.ncbi.nlm.nih.gov/10101239/

https://pubmed.ncbi.nlm.nih.gov/7766858/
https://pubmed.ncbi.nlm.nih.gov/15817635/

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。