Carboplatin

製品コードS1215 バッチS121512

化学情報

	Synonyms	NSC 241240, JM-8, CBDCA	Storage (From the date of receipt)	2 years 4°C/3 years -20°C(in the dark) powder
	化学式	C₆H₁₂N₂O₄Pt
	分子量	371.25	CAS No.	41575-94-4
Solubility (25°C)*	体外	Water	4.5 mg/mL warmed with 50ºC water bath (12.12 mM)
		Ethanol	Insoluble

* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Carboplatinは、A2780、SKOV-3、IGROV-1、およびHX62細胞において、DNAに結合し、細胞の修復メカニズムを妨害することにより、DNA synthesis阻害剤として作用します。溶液は不安定なので、使用直前に調製してください。DMSOは、プラチナ系薬剤の溶解には推奨されません。薬剤の不活性化を招く可能性があります。
in vitro	Carboplatin exhibits an inhibitory effect on cell proliferation in a human ovarian cancer cell line panel, including A2780, SKOV3, and IGROV-1 cells with IC50 of 6.1 μM, 12.4 μM and 2.2 μM, respectively. This compound also shows the anti-proliferative activities in lung carcinoid cell line, such as UMC-11, H727, and H835 cells with IC50 of 36.4 μM, 3.4 μM and 35.8 μM, respectively.
in vivo	In A2780 tumor xenografts, Carboplatin (60 mg/kg via i.p.) given as single agents shows a modest antitumor effect with the relative tumor volumes on day 6 of 8.4 compared to the control of 11.9, and the day 6 tumor weights relative to control (T/C) of 67%. For the VC8 (Brca2-deficient) xenografts, this compound treatment delays tumor growth and reduces tumor mass by 42% compared to the vehicle group.
特徴	This product is not recommended to be dissolved in dimethylsulfoxide (DMSO).

プロトコル(参考用のみ)

細胞アッセイ	細胞株	A2780, SKOV3, IGROV-1 and HX62
	濃度	0-200 μM
	反応時間	72 hours
	実験の流れ	3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assays: Exponentially growing A2780, SKOV3, IGROV-1 and HX62 ovarian cancer cells are plated in 96 well plates. A range of drug concentrations are added and the plates are incubated for 72 hours to allow for 3–4 doubling times. Each experiment is carried out in triplicate. Sulforhodamine B (SRB) assays: Exponentially growing A2780 cells are plated in 96 well microtitre plates. For experiments studying concomitant exposure, cells are exposed to increasing concentrations of both drugs for 96 hours. For experiments studying the effect of sequence of exposure to 17-AAG or this compound cells are exposed to increasing concentrations of 17-AAG or this chemical for 24 hours. A period of 24-hour exposure to the first agent is chosen so that the A2780 cells would be exposed to the first drug for at least one doubling time (18-24 hours). The cells are then washed with sterile phosphate buffered saline and the medium is replenished. Following this, the second drug (to which the cells are not exposed to in the first 24 hours) or medium is added for 96 hours. All experiments are carried out in triplicate. The results of combination studies are analyzed using the well-established principles of median effect analysis method. The effects of the combination are calculated using an in-house spreadsheet.
動物実験	動物モデル	The A2780 human ovarian cancer cell line is grown as a subcutaneous xenograft in female athymic NCr nude mice (nu/nu) in each flank.
	投薬量	≤60 mg/kg (When Carboplatin is dissolved in sodium chloride solution, it will induce physio-chemical and pharmacokinetic changes.)
	投与方法	Administered via i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/18193424/
https://pubmed.ncbi.nlm.nih.gov/21239354/
https://pubmed.ncbi.nlm.nih.gov/22778154/

https://pubmed.ncbi.nlm.nih.gov/15014014/
https://pubmed.ncbi.nlm.nih.gov/21868512/
https://pubmed.ncbi.nlm.nih.gov/24812268/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034078/

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カスタマーフィードバック

: Data from [PLoS One, 2012, 8, e72637]

: Data independently produced by , , Dr. Helen Sadik of Johns Hopkins University

: Data independently produced by , , Dr. Helen Sadik of Johns Hopkins University

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Non-canonical dihydrolipoyl transacetylase promotes chemotherapy resistance via mitochondrial tetrahydrofolate signaling [ Nat Commun, 2025, 16(1):8932]	PubMed: 41062483
Screening a living biobank identifies cabazitaxel as a strategy to combat acquired taxol resistance in high-grade serous ovarian cancer [ Cell Rep Med, 2025, 6(6):102160]	PubMed: 40466638
Profiling the Activity of the Potent and Highly Selective CDK2 Inhibitor BLU-222 Reveals Determinants of Response in CCNE1-Aberrant Ovarian and Endometrial Tumors [ Cancer Res, 2025, 10.1158/0008-5472.CAN-24-2360]	PubMed: 39945650
USP9X integrates TGF-β and hypoxia signalings to promote ovarian cancer chemoresistance via HIF-2α-maintained stemness [ Cell Death Dis, 2025, 16(1):312]	PubMed: 40246814
Cancer-metastasis-on-a-chip reveals efficacy of bevacizumab and siRNA in overcoming carboplatin resistance in SKOV3 ovarian cancer within a fibrotic metastatic microenvironment [ Mater Today Bio, 2025, 34:102240]	PubMed: 40917515
Low dose DNA methyltransferase inhibitors potentiate PARP inhibitors in homologous recombination repair deficient tumors [ Breast Cancer Res, 2025, 27(1):8]	PubMed: 39819384
ABCB1 confers resistance to carboplatin by accumulating stem-like cells in the G2/M phase of the cell cycle in p53null ovarian cancer [ Cell Death Discov, 2025, 11(1):132]	PubMed: 40175339
Homologous Repair-Deficient Pancreatic Cancer: Refined Targeting of DNA Damage Response is an Effective Therapeutic Strategy [ United Eur Gastroent, 2025, 13(7):1328-1342]	PubMed: 40823818
NAD+ Boosting Through NRH Supplementation Enhances Treatment Efficacy in EOC In Vitro [ Int J Mol Sci, 2025, 26(4)1719]	PubMed: 40004182
O 6-methylguanine DNA methyltransferase (MGMT) expression in U1242 glioblastoma cells enhances in vitro clonogenicity, tumor implantation in vivo, and sensitivity to alisertib-carboplatin combination treatment [ Front Cell Neurosci, 2025, 19:1552015]	PubMed: 40336841

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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