Carnosic acid

製品コードS3838 バッチS383802

印刷

化学情報

Chemical Structure Synonyms Salvin Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C20H28O4
分子量 332.43 CAS No. 3650-09-7
Solubility (25°C)* 体外 DMSO 66 mg/mL (198.53 mM)
Ethanol 66 mg/mL (198.53 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Carnosic acid (Salvin) is a phenolic diterpene, endowed with antioxidative and antimicrobial properties. It is increasingly used within food, nutritional health and cosmetics industries.
in vitro Carnosic acid (CA) would inhibit the cell viability of three CRC cell lines: Caco-2, HT29 and LoVo in a dose-dependent manner, with IC50 values in the range of 24-96 μM. CA induces cell death by apoptosis in Caco-2 line after 24 h of treatment and inhibits cell adhesion and migration, possibly by reducing the activity of secreted proteases such as urokinase plasminogen activator (uPA) and metalloproteinases (MMPs). CA downregulates the expression of COX-2 in Caco-2 cells at both the mRNA and protein levels[1]. CA induces antioxidant and anti-inflammatory effects in several cell types, as observed in both in vitro and in vivo experimental models. It exhibits the ability to promote mitochondrial protection in neural cells[2]. In vitro, CA suppresses Receptor Activator for Nuclear Factor-κ B Ligand (RANKL)- and Macrophage colony-stimulating factor (M-CSF)-induced osteoclastogenesis. The osteoclastic specific markers are inhibited by CA. Signal transduction studies show that CA significantly decreases the expression of molecules contributing to ROS and increases anti-oxidants. Additionally, CA inactivates the RANKL- and M-CSF-induced p38 mitogen activated protein kinases (MAPK), inhibits NF-κB phosphorylation, causing pro-inflammatory cytokines down-regulation. CA ameliorates acute promyelocytic leukemia cells through activating nuclear transcription factor E2-related factor 2 (Nrf2), which plays an essential role in cytoprotective responses to oxidative stress[3].
in vivo CA significantly down-regulates fasting blood glucose, glucose level in oral glucose tolerance test (OGTT) and insulin tolerance test (ITT), ameliorates CIA-induced bone loss, and reduces pro-inflammatory cytokines and reactive oxygen species (ROS) in db/db mice with arthritis induced by collagen-induced arthritis (CIA). CA could regulate ER stress to ameliorate disease progression. It can also cross the blood-brain barrier readily and exert its protective effects via its potent anti-oxidative role[3].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Human colon carcinoma cell lines, Caco-2, HT29 and LoVo
濃度 0 to 388 μM
反応時間 24 h
実験の流れ Cells (1×104) are seeded in 96-well microplates in complete medium. After 48 h, cells are washed twice with PBS and treated with RE, RA and CA (concentration range from 0 to 388 μM) in complete medium for 24 h. Cell viability is assessed.
動物実験 動物モデル male C57BL/KsJ-db/db (db/db) mice
投薬量 30 mg/kg and 60 mg/kg
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Pilot-Scale Antioxidant Dipping of Herring (Clupea harengus) Co-products to Allow Their Upgrading to a High-Quality Mince for Food Production [ ACS Sustain Chem Eng, 2023, 11(12):4727-4737] PubMed: 37013165
NQO1 Is a Determinant for Cellular Sensitivity to Anti-Tumor Agent Napabucasin [ Am J Cancer Res, 2020, 1;10(5):1442-1454] PubMed: 32509390
Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species [ J Reprod Dev, 2020, 10.1262/jrd.2020-086] PubMed: 33055461

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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